Difference between revisions of "Team:Goettingen/Description"

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<h1> '''About our Projec'''t </h1>
  
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<h2> Project Description </h2>
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<h2> Our project </h2>
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The '''iGEM team Goettingen 2015''' is currently developing “'''Flexosome'''”
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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It is our '''enzymatic penknife''': a customizable complex for more efficient and '''synergistic multi-enzymatic processes'''. It consists of a '''scaffoldin, dockerins''' and '''exchangeable enzymes'''.
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The enzymes of interest can be attached to the scaffoldin via the dockerin stations and once attached complete the reactions.
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<h2> '''The first step''' </h2>
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<h5>What should this page contain?</h5>
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Right now the team is working on a proof of concept. Our first Flexosome will contain '''phosphatase, estherase''' and '''lipase''' found in metagenomic libraries along with a fluorescent reporter gene. Once our E. coli is able to express and produce this functional Flexosome, this will open the door for more complex and specific applications.
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<h2>'''The inspiration''' </h2>
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<li> A clear and concise description of your project.</li>
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We studied the natural '''cellulosome structure''', in which different cellulotytic enzymes are assembled into a bigger scaffoldin via dockerin stations, leading to optimized cellulose degradation. The interaction between scaffoldin and dockerins has been proven to be '''strong and stable''', this gave us the ideal platform to design our '''“Flexosome”''' device.
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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Traditionally cellulosome engineering is thought for optimization of cellulose degradation, but we don’t have to stop there! A much broader range of enzymes are actively being discovered every year. That is why we intend to combine the scaffoldin protein from cellulolytic bacterium with varying and exchangeable enzymes. The result: a very '''versatile, stable and innovative tool'''!
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<li>References and sources to document your research.</li>
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<h2> '''The goal''' </h2>
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We want to achieve a protein construct which will be able to ensure a '''high local concentration''' of enzymes and '''catalyse multiple enzymatic processes''' at one site, making the product of one process the substrate of the next on the scaffoldin. Ultimately, the construct will not only '''increase efficiency''' of the reactions but also be '''fully customisable''' for any kind of enzymatic process.
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<h4>Advice on writing your Project Description</h4>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h4>References</h4>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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<h4>Inspiration</h4>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Revision as of 17:18, 14 July 2015

About our Project

Our project

The iGEM team Goettingen 2015 is currently developing “Flexosome” It is our enzymatic penknife: a customizable complex for more efficient and synergistic multi-enzymatic processes. It consists of a scaffoldin, dockerins and exchangeable enzymes. The enzymes of interest can be attached to the scaffoldin via the dockerin stations and once attached complete the reactions.

The first step

Right now the team is working on a proof of concept. Our first Flexosome will contain phosphatase, estherase and lipase found in metagenomic libraries along with a fluorescent reporter gene. Once our E. coli is able to express and produce this functional Flexosome, this will open the door for more complex and specific applications.

The inspiration

We studied the natural cellulosome structure, in which different cellulotytic enzymes are assembled into a bigger scaffoldin via dockerin stations, leading to optimized cellulose degradation. The interaction between scaffoldin and dockerins has been proven to be strong and stable, this gave us the ideal platform to design our “Flexosome” device. Traditionally cellulosome engineering is thought for optimization of cellulose degradation, but we don’t have to stop there! A much broader range of enzymes are actively being discovered every year. That is why we intend to combine the scaffoldin protein from cellulolytic bacterium with varying and exchangeable enzymes. The result: a very versatile, stable and innovative tool!

The goal

We want to achieve a protein construct which will be able to ensure a high local concentration of enzymes and catalyse multiple enzymatic processes at one site, making the product of one process the substrate of the next on the scaffoldin. Ultimately, the construct will not only increase efficiency of the reactions but also be fully customisable for any kind of enzymatic process.