Difference between revisions of "Team:Paris Saclay/Notebook/July/9"
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Revision as of 10:17, 15 July 2015
Contents
Wednesday 8th July
Lab Work
Transformation
by Coralie
Ligation product:
- J23101 + I13504
- J23106 + I13504
- J23117 + I13504
On LB + Ampicillin 100ug/mL Incubation ON, 37°C
"by Johan and Seong Koo"
R0051 On LB + Ampicillin 100ug/mL Incubation ON, 37°C
Plasmid Rehydratation :
by Johan and Audrey
Biobricks:
- S03518
- B0030
- B0015
- K1399005
Digestion
by Pauline and Audrey
Plasmid with promotor: J23101 10uL of our plasmid with promotor 1uL SpeI 1uL PstI 2uL buffer 10x FD
Plasmid with gene: COO40 and K115017 10uL of our plasmid with gene 1uL XbaI 1uL PstI 2uL buffer 10x FD
After this step, we separate the sequence we need from the sequence we don't with electrophoresis
Electrophoresis
by Pauline and Audrey
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V
Quantification
by Pauline and Audrey We quantify with the electrophoresis gel: C0040: 10ng/uL J23101: 20ng/uL K115017: we don't see enough of fluorescent to quantify the biobrick. We decide to amplify this one by PCR.
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Audrey, Coralie, Pauline