Difference between revisions of "Team:Paris Saclay/Notebook/July/7"

(→‎Tuesday 7th July)
Line 4: Line 4:
 
''by Coralie, Johan, Seong Koo, Audrey, Pauline''
 
''by Coralie, Johan, Seong Koo, Audrey, Pauline''
  
−
Biobricks verification: K115017; C0040; I13602; J23101; J23117; J23106; I13504; K1493504
+
=====Biobricks verification:======
−
Digestion by NotI :
+
 
 +
*K115017
 +
*C0040
 +
*I13602
 +
*J23101
 +
*J23117
 +
*J23106
 +
*I13504
 +
*K1493504
 +
 
 +
Extraction with Kit nucleospin
 +
 
 +
=====Digestion by NotI :=====
 +
 
 
Stock solution preparation : 124uL H2O, 16uL buffer 10x, 4uL NotI
 
Stock solution preparation : 124uL H2O, 16uL buffer 10x, 4uL NotI
 +
 
We use 18uL of this solution with 2uL plasmid solution
 
We use 18uL of this solution with 2uL plasmid solution
 +
 
Digestion is process during 1h30 at 37°C
 
Digestion is process during 1h30 at 37°C
  
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Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET
 
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET
 
Migration 0,06A 80V
 
Migration 0,06A 80V
 +
  
 
====New culture====
 
====New culture====

Revision as of 10:21, 15 July 2015

Tuesday 7th July

Back in Lab

Verification of our plasmid

by Coralie, Johan, Seong Koo, Audrey, Pauline

Biobricks verification:=
  • K115017
  • C0040
  • I13602
  • J23101
  • J23117
  • J23106
  • I13504
  • K1493504

Extraction with Kit nucleospin

Digestion by NotI :

Stock solution preparation : 124uL H2O, 16uL buffer 10x, 4uL NotI

We use 18uL of this solution with 2uL plasmid solution

Digestion is process during 1h30 at 37°C

Electrophoresis

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V


New culture

by Pauline, Audrey

New liquid cultures of:

  • K1493504

2x 5ml LB + 10μl Chloramphenicol + 1 bacteria colonies. We incubate cultures at 37°C.


Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Audrey, Coralie, Pauline