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| − | <div align="center"><h2>A BLUE E. COLI IS A HAPPY E. COLI</h2></div>
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| − | <h2>ANTHOCYANINS</h2>
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| − | A class of pigmented (blue, red and purple) compounds which are members of the flavonoid group of phytochemicals, possessing aromatics structures with bioactive properties. They are produced through a complex biochemical pathway involving the actions of multiple enzymes, as seen in Figure 1. The initial substrate of this pathway is phenylalanine, which is a common amino acid utilized by all living organisms. Some natural sources of anthocyanins include honey, fruits and vegetables, nuts, cocoa and olive oil.<br>
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| − | Anthocyanin compounds are free radical scavengers which sequester harmful reactive oxygen species, thereby acting as antioxidants. Anthocyanins provide a variety of physiological benefits to humans. Some such benefits include:<br>
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| − | • Prevention of DNA cleavage<br>
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| − | • Regulation of the immune system<br>
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| − | • Prevention of cardiovascular disease, cognitive decline and cancer<br>
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| − | • Enhanced visual acuity<br>
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| − | • Regulation of lipid degradation<br>
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| − | <h2>THE OBJECTIVE</h2>
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| − | The mass production of anthocyanin compounds may have significant implications in the treatment and prevention of a large number of diseases and disorders. Therefore the goal of this project is to produce a recombinant strain of Escherichia coli which is capable of expressing the anthocyanin biosynthetic pathway. This will lead to the visualization of a blue phenotype when the strain is cultured. This project is modelled on a previous application of synthetic biology involving the expression of the lycopene pathway in E.coli by Klein-Marcuschamer et al (2007).
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| − | <h2>REFERENCES</h2>
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| − | Klein-Marcuschamer, D., Kumaran Ajikumar, P., & Stephanopoulos, G. (2007). Engineering
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| − | microbial cell factories for the biosynthesis of isoprenoid molecules: Beyond lycopene.
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| − | Trends in Biotechnology, 25(9). 417-424. doi:10.1016/j.tibtech.2007.07.006<br>
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| − | Lila, M.A. (2004). Anthocyanins and human health: An in vitro investigative approach. Journal
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| − | of Biomedicine and Biotechnology, 2004(5). 306-313. doi: 10.1155/S111072430440401X
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| | + | <img src="https://static.igem.org/mediawiki/2015/5/53/Team_Dalhousie_Halifax_NS_Project.jpg" alt="" usemap="#Map" /> |
| | + | <map name="Map" id="Map"> |
| | + | <area alt="" title="Project" href="https://2015.igem.org/Team:Dalhousie_Halifax_NS/Why" shape="rect" coords="38,16,435,129" /> |
| | + | <area alt="" title="Blueberries" href="https://2015.igem.org/Team:Dalhousie_Halifax_NS/Blueberries" shape="rect" coords="331,193,774,293" /> |
| | + | <area alt="" title="Project" href="https://2015.igem.org/Team:Dalhousie_Halifax_NS/Why" shape="rect" coords="40,400,528,553" /> |
| | + | <area alt="" title="Anthocyanins" href="https://2015.igem.org/Team:Dalhousie_Halifax_NS/What" shape="rect" coords="387,615,951,845" /> |
| | + | </map> |
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| | + | <img src="https://static.igem.org/mediawiki/2015/d/dd/Team_Dalhousie_Halifax_NS_Line.jpg"> |
| | + | <img src="https://static.igem.org/mediawiki/2015/c/c3/Team_Dalhousie_Halifax_NS_Footer.jpg" alt="" usemap="#Map2" /> |
| | + | <map name="Map2" id="Map2"> |
| | + | <area alt="" title="Contact us" href="https://2015.igem.org/Team:Dalhousie_Halifax_NS/contact" shape="rect" coords="364,25,621,187" /> |
| | + | </map> |
| | + | </div> |
| | </html> | | </html> |
