Revision as of 14:23, 15 July 2015

Tuesday 7th July

by Coralie, Johan, Seong Koo, Audrey, Pauline

With the Nucleospin Kit from Macherey Nagel

Stock solution preparation :

We use 18µL from this solution with 2µL plasmid solution

Incubation 1h30 at 37°C

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

by Pauline, Audrey

New liquid culture of:

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Members present:

@@ Line 1: / Line 1: @@
 =Tuesday 7th July=
 ==Back in Lab==
-====Verification of our plasmid====
+====Verification of our plasmids====
 ''by Coralie, Johan, Seong Koo, Audrey, Pauline''
@@ Line 15: / Line 15: @@
 *K1493504
-Extraction with Kit nucleospin
+=====Extraction=====
-=====Digestion by NotI :=====
+With the Nucleospin Kit from Macherey Nagel
-Stock solution preparation : 124uL H2O, 16uL buffer 10x, 4uL NotI
+=====Digestion by NotI: =====
-We use 18uL of this solution with 2uL plasmid solution
+Stock solution preparation :
+* 124µL H2O
+* 16µL buffer FastDigest 10x
+* 4µL NotI
-Digestion is process during 1h30 at 37°C
+We use 18µL from this solution with 2µL plasmid solution
-====Electrophoresis====
+Incubation 1h30 at 37°C
+=====Electrophoresis=====
 ''by Johan, Audrey, Pauline''
@@ Line 35: / Line 40: @@
 ''by Pauline, Audrey''
-New liquid cultures of:
+New liquid culture of:
 *K1493504
-x 5ml LB + 10μl Chloramphenicol + 1 bacteria colonies. We incubate cultures at 37°C.
+x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.
+We incubate cultures at 37°C, ON.