Difference between revisions of "Team:Manchester-Graz/Project"
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<div id="pictureleft"><img src="https://static.igem.org/mediawiki/2015/e/ef/Manchester-Graz_Pathway.jpg" alt="Pathway" width="300"> <br><b> Fig 2 </b>Novel dopamine expressing pathways </div> | <div id="pictureleft"><img src="https://static.igem.org/mediawiki/2015/e/ef/Manchester-Graz_Pathway.jpg" alt="Pathway" width="300"> <br><b> Fig 2 </b>Novel dopamine expressing pathways </div> | ||
− | <p style="text-align:justify">The Manchester section of the team are working on L-DOPA and dopamine biosynthesis in <i>E. coli </i>BL21 and Nissle 1917 via various enzyme pathways. The focus is on three enzyme pathways, the main one being the conversion of L-tyrosine to L-DOPA via tyrosine hydroxylase and tyrosinase. In addition we are also synthesising dopamine in two different ways using aromatic amino acid decarboxylase, cytochrome P450 and transaminase. Although the primary goal would be to implement the L-DOPA synthesis within patients, we also aim to create a greener, more efficient way of the industrial synthesis of each of the above products using our modified bacteria. (Figure 2) </p> | + | <p style="text-align:justify"><br>The Manchester section of the team are working on L-DOPA and dopamine biosynthesis in <i>E. coli </i>BL21 and Nissle 1917 via various enzyme pathways. The focus is on three enzyme pathways, the main one being the conversion of L-tyrosine to L-DOPA via tyrosine hydroxylase and tyrosinase. In addition we are also synthesising dopamine in two different ways using aromatic amino acid decarboxylase, cytochrome P450 and transaminase. Although the primary goal would be to implement the L-DOPA synthesis within patients, we also aim to create a greener, more efficient way of the industrial synthesis of each of the above products using our modified bacteria. (Figure 2) </p> |