Difference between revisions of "Team:China Tongji/Protocol"

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                    <p>Breast cancer is the most common malignancy in women and constitutes 18% of all female cancers (Jemal et al., 2002) Although there has been a slight decrease in mortality in breast cancer patients (Schiffman et al., 2002), it is not uncommon for even early-stage breast cancer to metastasize. Therefore, novel therapeutic strategies are constantly being pursued (Bange et al., 2001). It has been reported that some bacterial species preferentially replicate and accumulate within tumors. At critical cell densities, the binding of a regulator protein to the signal leads to the switch on of genes controlled by QS and therefore a coordinated population response Moreover, they possess certain advantageous features such as motility, capacity to simultaneously carry and express multiple therapeutic proteins. Its elimination by antibiotics makes it a promising new strategy in cancer treatment. Azurin is a copper-containing redox protein with low molecular Weight which can efficiently induce cell apoptosis by raising the intracellular levels of p53 and Bax. It has been found that Azurin receptors are hyperexpressed on the surfaces of cancer cells relative to their expression on the surfaces of normal cells. We do intend to modify genetic material of bacteria specie that localize and induce expression of Azurin in presence of tumor only.
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                    <p>Using hardware devices in wet lab can help scientists by many different ways. It can help in measurement and tracking biological components. Besides it can be used to create more accurate tools and simplify complexes process. Sensors used in hardware devices have a lot of factors that can be detected like pH value, temperature, charge and a lot of other factors. We are aiming to create hardware device that test our biological system by measuring the death rate of the cancer cells after induction of Azurin gene. This device can be used separately to test apoptosis rate in any sample population.</p>
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        Copyright © iGEM Cairo Egypt Team 2015
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Revision as of 22:34, 16 July 2015

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