Difference between revisions of "Team:Paris Saclay/Notebook/July/9"
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====Quantification==== | ====Quantification==== | ||
''by Pauline and Audrey'' | ''by Pauline and Audrey'' | ||
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| + | Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET | ||
| + | Migration 0,06A 80V | ||
We quantify with the electrophoresis gel: | We quantify with the electrophoresis gel: | ||
* C0040: 10ng/µL | * C0040: 10ng/µL | ||
Revision as of 09:39, 17 July 2015
Contents
Thursday 9th July
Lab Work
Transformation
by Coralie
Ligation product:
- J23101 + I13504
- J23106 + I13504
- J23117 + I13504
On LB + Ampicillin 100ug/mL Incubation ON, 37°C
by Johan and Seong Ko
R0051 On LB + Ampicillin 100ug/mL Incubation ON, 37°C
Plasmid Rehydratation :
by Johan and Audrey
Biobricks:
- S03518
- B0030
- B0015
- K1399005
Digestion
by Pauline and Audrey
Plasmid with promotor: J23101
- 10µL of our plasmid with promotor
- 1µL SpeI
- 1µL PstI
- 2µL buffer 10x FD
Plasmid with gene: COO40 and K115017
- 10µL of our plasmid with gene
- 1µL XbaI
- 1µL PstI
- 2µL buffer 10x FD
After this step, we separate the sequence we need from the sequence we don't with electrophoresis
Electrophoresis
by Pauline and Audrey
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V
Quantification
by Pauline and Audrey
Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V We quantify with the electrophoresis gel:
- C0040: 10ng/µL
- J23101: 20ng/µL
- K115017: we don't see enough of fluorescent to quantify the biobrick. We decide to amplify this one by PCR.
Members present:
- Instructors and advisors: Alice.
- Students: Johan, Seong Koo, Audrey, Coralie, Pauline