Difference between revisions of "Team:Kent/Notebook"

Line 4: Line 4:
  
 
<html>
 
<html>
 +
 +
<div>
 +
<script type="text/javascript">
 +
$(document).ready(function(e) {
 +
t = $('.fixed').offset().top;
 +
mh = $('.main').height();
 +
fh = $('.fixed').height();
 +
$(window).scroll(function(e){
 +
s = $(document).scrollTop();
 +
if(s > t - 100){
 +
$('.fixed').css('position','fixed');
 +
if(s + fh - 2000 > mh){
 +
$('.fixed').css('top',mh-s-fh+'px');
 +
}
 +
}else{
 +
$('.fixed').css('position','');
 +
}
 +
})
 +
});
 +
</script>
 +
 +
 +
 +
<div class="box">
 +
 +
<div class="sub">
 +
<div class="fixed">
 +
<style type="text/css">
 +
li{margin-bottom:0px;}
 +
.cont ul,li{list-style: none;} 
 +
.cont ul {padding: 0; margin: 0;text-align:center;} 
 +
.cont .hmain{background-color:#0babe7 ;width: 220px;font-size:16px;float: left;border-top:#fff thin solid;border-bottom:#fff thin solid;} 
 +
 
 +
.cont a {text-decoration: none; /* padding-left: 10px;*/  display: block;  display: inline-block; width: 220px;padding-top: 7px;padding-bottom: 7px;} 
 +
.cont .hmain a{color:#fff;  }
 +
.cont .hmain a:hover{color:#0babe7;background:#fff;border-top:#0babe7 thin solid;border-bottom:#0babe7 thin solid;  } 
 +
.cont .hmain ul {display: none;} 
 +
 
 +
</style>
 +
 +
<script type="text/javascript">
 +
$(document).ready(function(){ 
 +
    $(".hmain").hover(function(){ 
 +
        $(this).children("ul").slideDown(); 
 +
        changeIcon($(this).children("a")); 
 +
    },function(){ 
 +
        $(this).children("ul").slideUp(); 
 +
        changeIcon($(this).children("a")); 
 +
    }); 
 +
});
 +
</script>
 +
 +
 +
<div class="cont" style="margin-top:20px;">
 +
        <ul>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/Team:Tianjin/Kent">June,2015</a>
 +
                <ul>
 +
                    <li> 
 +
<style type="text/css">
 +
.table1{width:220px;}
 +
.table1 td{color:#d2d2d2;background-color: #fff; margin:1px;width:27px;height:27px;text-align:center;}
 +
.table1 #alt{color:#fff;background-color: #4ccbfa; border:1px solid #fff;width:27px;height:27px;text-align:center;}
 +
.table1 th{font-size:14px;text-align:left;background-color:#d2d2d2;color:#fff; border:1px solid #fff;width:27px;height:27px;text-align:center;}
 +
.table1 #alt a{color:#fff;background-color: #4ccbfa;width:27px;height:27px;text-align:center;margin:0px;padding:0px;border:none;}
 +
</style>
 +
 +
                   
 +
<table class="table1">
 +
<tr>
 +
<th>Sun</th>
 +
<th>Mon</th>
 +
<th>Tue</th>
 +
<th>Wed</th>
 +
<th>Thu</th>
 +
<th>Fri</th>
 +
<th>Sat</th>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>1</td>
 +
</tr>
 +
 +
<tr>
 +
<td>2</td>
 +
<td>3</td>
 +
<td>4</td>
 +
<td>5</td>
 +
<td>6</td>
 +
<td>7</td>
 +
<td>8</td>
 +
</tr>
 +
 +
<tr>
 +
<td>9</td>
 +
<td>10</td>
 +
<td>11</td>
 +
<td>12</td>
 +
<td>13</td>
 +
<td>14</td>
 +
<td>15</td>
 +
</tr>
 +
 +
<tr>
 +
<td>16</td>
 +
<td>17</td>
 +
<td>18</td>
 +
<td>19</td>
 +
<td>20</td>
 +
<td>21</td>
 +
<td>22</td>
 +
</tr>
 +
 +
<tr>
 +
<td>23</td>
 +
<td>24</td>
 +
<td>25</td>
 +
<td>26</td>
 +
<td>27</td>
 +
<td>28</td>
 +
<td>29</td>
 +
</tr>
 +
 +
<tr>
 +
<td>30</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
</tr>
 +
 +
 +
</table>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/Team:Kent/Notebook">July,2015</a>
 +
                <ul>
 +
                    <li>
 +
             
 +
<table class="table1">
 +
<tr>
 +
<th>Sun</th>
 +
<th>Mon</th>
 +
<th>Tue</th>
 +
<th>Wed</th>
 +
<th>Thu</th>
 +
<th>Fri</th>
 +
<th>Sat</th>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td>1</td>
 +
<td>2</td>
 +
<td>3</td>
 +
<td>4</td>
 +
<td>5</td>
 +
<td>6</td>
 +
</tr>
 +
 +
<tr>
 +
<td>7</td>
 +
<td>8</td>
 +
<td>9</td>
 +
<td>10</td>
 +
<td>11</td>
 +
<td>12</td>
 +
<td>13</td>
 +
</tr>
 +
 +
<tr>
 +
<td>14</td>
 +
<td>15</td>
 +
<td>16</td>
 +
<td>17</td>
 +
<td>18</td>
 +
<td>19</td>
 +
<td>20</td>
 +
</tr>
 +
 +
<tr>
 +
<td>21</td>
 +
<td>22</td>
 +
<td>23</td>
 +
<td>24</td>
 +
<td>25</td>
 +
<td>26</td>
 +
<td>27</td>
 +
</tr>
 +
 +
<tr>
 +
<td>28</td>
 +
<td>29</td>
 +
<td>30</td>
 +
<td>31</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
</tr>
 +
 +
 +
</table>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/Team:Tianjin/Kent">August,2015</a>
 +
                <ul>
 +
                    <li>
 +
         
 +
<table class="table1">
 +
<tr>
 +
<th>Sun</th>
 +
<th>Mon</th>
 +
<th>Tue</th>
 +
<th>Wed</th>
 +
<th>Thu</th>
 +
<th>Fri</th>
 +
<th>Sat</th>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>1</td>
 +
<td>2</td>
 +
<td>3</td>
 +
</tr>
 +
 +
<tr>
 +
<td>4</td>
 +
<td>5</td>
 +
<td>6</td>
 +
<td>7</td>
 +
<td>8</td>
 +
<td>9</td>
 +
<td>10</td>
 +
</tr>
 +
 +
<tr>
 +
<td>11</td>
 +
<td>12</td>
 +
<td>13</td>
 +
<td>14</td>
 +
<td>15</td>
 +
<td>16</td>
 +
<td>17</td>
 +
</tr>
 +
 +
<tr>
 +
<td>18</td>
 +
<td>19</td>
 +
<td>20</td>
 +
<td>21</td>
 +
<td>22</td>
 +
<td>23</td>
 +
<td>24</td>
 +
</tr>
 +
 +
<tr>
 +
<td>25</td>
 +
<td>26</td>
 +
<td>27</td>
 +
<td>28</td>
 +
<td>29</td>
 +
<td>30</td>
 +
<td>31</td>
 +
</tr>
 +
 +
</table>
 +
 +
 +
</div>
 +
 +
 +
  
 
<style>
 
<style>

Revision as of 10:06, 17 July 2015


iGEM Kent 2015

  • June,2015
    • Sun Mon Tue Wed Thu Fri Sat
                  1
      2 3 4 5 6 7 8
      9 10 11 12 13 14 15
      16 17 18 19 20 21 22
      23 24 25 26 27 28 29
      30            
  • July,2015
    • Sun Mon Tue Wed Thu Fri Sat
        1 2 3 4 5 6
      7 8 9 10 11 12 13
      14 15 16 17 18 19 20
      21 22 23 24 25 26 27
      28 29 30 31      
  • August,2015
    • Sun Mon Tue Wed Thu Fri Sat
              1 2 3
      4 5 6 7 8 9 10
      11 12 13 14 15 16 17
      18 19 20 21 22 23 24
      25 26 27 28 29 30 31

Notebook

June

M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30

July

M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 2930 31

August

M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 2627 28 29 30
31

September

M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30



















Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved

    • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight
  • Meeting with advisors to discuss progress

    • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid

    • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348

    • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis

    • Day 6 Wet lab 29/06/15

    Transforming linear PSB1C3

    Day 7 Wet lab 30/06/15

  • Miniprep of PSB1A3 plasmid
  • Gel electrophoresis of a large quantity of PSB1C3
  • Meeting with advisors to discuss progress

    • Day 8 Wet lab 01/07/15

  • Gel electrophoresis of the purified DNA extracted
  • Transformation of pSB1A3 circular plasmid to VS45 cells (competent)
  • Digest of all pSB1C3 plasmids

    • Day 9 Wet lab 02/07/15

  • Competent cells transformation efficiency
  • Mini-prep of 1MS340 to get pSB1C3 plasmid
  • Gel extraction of big digest
  • Quantification of the digest. Added sample buffer to each digest

    • Day 10 Wet lab 03/07/15

  • Transformation efficiency

    • Day 11 Wet lab 06/07/15

  • TOP10 cells containing pSB1A3 with limonene synthase were cultured overnight on AMP plates
  • Colonies of VS45 containing pSB1A3 were patched onto chloramphenicol plates
  • Overnight digest of miniprepped pSBIC3 using ECORI and PSTl

    • Day 12 Wet lab 07/07/15

  • Agarose gel of overnight digest - no bands were visible
  • Set up overnight cultures of pSBIC3 and pSBIA3 to be miniprepped the next day
  • Set up an overnight digest of pSBIC3

    • Day 13 Wet lab 08/07/15

  • Agarose gel of overnight digest - no bands were visible
  • Miniprep of pSBIA3 and pSBIC3
  • Overnight digest of pSBIA3 and pSBIC3

    • Day 14 Wet lab 09/07/15

  • Agarose gel of digested pSBIA3 and pSBIC3 - no bands were visible
  • Overnight cultures of MS349 containing pSBIA3 LIMS and MS340 containing pSBIC3 were set up, ready to be miniprepped

    • Day 15 Wet lab 10/07/15

  • Miniprep of plasmids pSBIA3 and pSBIC3

    • Day 16 Wet lab 13/07/15

  • Overnight cultures of MS349 containing pSBIA3 LIMS and MS340 containing pSBIC3 were set up, ready to be miniprepped
  • Overnight digest of miniprepped plasmids pSBIA3 and pSBIC3 using the enzymes ECORI and PSTI

    • Day 17 Wet lab 14/07/15

  • Agarose gel of digested plasmids pSBIA3 and pSBIC3 - it finally worked!!!
  • Miniprep of overnight cultures
  • Gel extraction of both plasmids
  • Analytical gel using Hyperladder I to quantify the plasmid
  • Overnight cultures of Top10 cells containing PVS72

    Day 18 Wet lab 15/07/15

  • Miniprepped PVS72
  • Transformation of VS45 with PVS72
  • Made LB plates with Chloramphenicol and Ampicillin
  • Plated the transformed VS45 cells on the Chloramphenicol/Ampicillin plates
  • Agarose gel of leftover digested pSBIA3 and pSBIC3 from 14/07/15
  • Gel extraction of pSBIA3 and pSBIC3

    • Day 19 Wet lab 16/07/15

  • Counted the overnight colonies of VS45 with PVS72
  • Calculated the transformation efficiency