Difference between revisions of "Team:Paris Saclay/Notebook/July/7"

(Tuesday 7th July)
m
Line 1: Line 1:
 +
{{Team:Paris_Saclay/notebook_header}}
 
=Tuesday 7th July=
 
=Tuesday 7th July=
==Back in Lab==
+
==Labwork==
====Verification of our plasmids====
+
===Verification of our plasmids===
 
''by Coralie, Johan, Seong Koo, Audrey, Pauline''
 
''by Coralie, Johan, Seong Koo, Audrey, Pauline''
  
=====Biobricks verification:=====  
+
====Biobricks verification====
 
+
 
*K115017
 
*K115017
 
*C0040
 
*C0040
Line 14: Line 14:
 
*I13504
 
*I13504
  
=====Extraction=====
+
====Extraction====
 
+
 
With the Nucleospin Kit from Macherey Nagel
 
With the Nucleospin Kit from Macherey Nagel
  
=====Digestion by NotI: =====
+
====Digestion by NotI====
 
+
 
Stock solution preparation :  
 
Stock solution preparation :  
 
* 124µL H2O
 
* 124µL H2O
Line 29: Line 27:
 
Incubation 1h30 at 37°C
 
Incubation 1h30 at 37°C
  
=====Electrophoresis=====
+
====Electrophoresis====
 
''by Johan, Audrey, Pauline''
 
''by Johan, Audrey, Pauline''
  
Line 35: Line 33:
 
Migration 0,06A 80V
 
Migration 0,06A 80V
  
 
+
===New culture===
====New culture====
+
 
''by Pauline, Audrey''
 
''by Pauline, Audrey''
  
Line 44: Line 41:
 
2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.  
 
2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.  
 
We incubate cultures at 37°C, ON.
 
We incubate cultures at 37°C, ON.
 
  
 
'''Members present:'''
 
'''Members present:'''
 
* Instructors and advisors: Alice.
 
* Instructors and advisors: Alice.
 
* Students: Johan, Seong Koo, Audrey, Coralie, Pauline
 
* Students: Johan, Seong Koo, Audrey, Coralie, Pauline
 +
 +
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 19:43, 21 July 2015


Tuesday 7th July

Labwork

Verification of our plasmids

by Coralie, Johan, Seong Koo, Audrey, Pauline

Biobricks verification

  • K115017
  • C0040
  • I13602
  • J23101
  • J23117
  • J23106
  • I13504

Extraction

With the Nucleospin Kit from Macherey Nagel

Digestion by NotI

Stock solution preparation :

  • 124µL H2O
  • 16µL buffer FastDigest 10x
  • 4µL NotI

We use 18µL from this solution with 2µL plasmid solution

Incubation 1h30 at 37°C

Electrophoresis

by Johan, Audrey, Pauline

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5uL of BET Migration 0,06A 80V

New culture

by Pauline, Audrey

New liquid culture of:

  • K1493504

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Members present:

  • Instructors and advisors: Alice.
  • Students: Johan, Seong Koo, Audrey, Coralie, Pauline

Back to the calendar