Latest revision as of 19:59, 21 July 2015

Wednesday 15th July

Lab Work

Observation of our plates

by Coralie and Johan

Transformations from the 07/10/2015

We can observe that BBa_S03518, BBa_B0030 and BBa_B0015 are OK. But BBa_K1399005 didn't grow. We think that we make a mistake when we rehydrated the plasmid.

Transformations from the 07/09/2015

We can observe that BBa_J23101 + BBa_I13504 and BBa_J23106 + BBa_I13504 are yellow when we expose them to the white light. But BBa_J23117 + BBa_I13504 don't seem to be yellow on the white light.

New culture

by Coralie, Johan and Seong Koo

New liquid culture of:

Transformations from the 07/09/2015:
- BBa_J23101 + BBa_I13504
- BBa_J23106 + BBa_I13504
- BBa_J23117 + BBa_I13504

BBa_B0015
BBa_B0030
BBa_S03518

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Ligation

by Pauline

Reaction mix:

BBa_J23101: 1µL
BBa_K115017: 10µL
Buffer (10x): 2µL
Ligase: 1µL
H2O: 6µL

Incubation 3h at 4°C

Transformation

by Seong Koo

BBa_K139905
BBa_K1399019
BBa_K1399023

Ligation product:

BBa_J23101 + BBa_K115017 = BBa_K1707000

Plasmid extraction

BBa_R0051

Culture form the 07/10/2015 We put back in culture 200µL of this one in 5mL of LB + Ampicilline

With the Nucleospin Kit from Macherey Nagel

Members present:

Instructors and advisors: Alice.
Students: Johan, Seong Koo, Coralie, Pauline

Back to the calendar

@@ Line 1: / Line 1: @@
+{{Team:Paris_Saclay/notebook_header}}
 =Wednesday 15th July=
-==Back in Lab==
+==Lab Work==
+===Observation of our plates===
-====Observation of our plates====
 ''by Coralie and Johan''
 *Transformations from the 07/10/2015
-We can observe that S03518, B0030 and B0015 are Ok.
+We can observe that BBa_S03518, BBa_B0030 and BBa_B0015 are OK.
-But K1399005 didn't grow. We think that we make a mistake when we rehydrated the plasmid.
+But BBa_K1399005 didn't grow. We think that we make a mistake when we rehydrated the plasmid.
 *Transformations from the 07/09/2015
-We can observe that J23101 + I13504 and J23106 + I13504 are yellow when we expose them to the white light.
+We can observe that BBa_J23101 + BBa_I13504 and BBa_J23106 + BBa_I13504 are yellow when we expose them to the white light.
-But J23117 + I13504 don't seem to be yellow on the white light.
+But BBa_J23117 + BBa_I13504 don't seem to be yellow on the white light.
-====New culture====
+===New culture===
 ''by Coralie, Johan and Seong Koo''
 New liquid culture of:
 *Transformations from the 07/09/2015:
-**J23101 + I13504
+** BBa_J23101 + BBa_I13504
-**J23106 + I13504
+** BBa_J23106 + BBa_I13504
-**J23117 + I13504
+** BBa_J23117 + BBa_I13504
-*B0015
-*B0030
-*S03518
+* BBa_B0015
+* BBa_B0030
+* BBa_S03518
 x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony.
 We incubate cultures at 37°C, ON.
+===Ligation===
-=====Ligation:=====
 ''by Pauline''
 Reaction mix:
-* J23101: 1µL
+* BBa_J23101: 1µL
-* K115017: 10µL
+* BBa_K115017: 10µL
 * Buffer (10x): 2µL
 * Ligase: 1µL
@@ Line 44: / Line 41: @@
 Incubation 3h at 4°C
-=====Transformation=====
+===Transformation===
 ''by Seong Koo''
-Biobricks:
+* BBa_K139905
-* K139905
+* BBa_K1399019
-* K1399019
+* BBa_K1399023
-* K1399023
 Ligation product:
-* J23101 + K115017
+* BBa_J23101 + BBa_K115017 = BBa_K1707000
+===Plasmid extraction===
+* BBa_R0051
+Culture form the 07/10/2015
+We put back in culture 200µL of this one in 5mL of LB + Ampicilline
-=====Plasmid extraction =====
-Biobrick: R0051
-Culture form the 07/10/2015
 With the Nucleospin Kit from Macherey Nagel
 '''Members present:'''
 * Instructors and advisors: Alice.
 * Students: Johan, Seong Koo, Coralie, Pauline
+{{Team:Paris_Saclay/notebook_footer}}

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