Difference between revisions of "Team:Gifu/project/"
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<h4> <font size="5" face="Century"> Project Description </font> </h4> | <h4> <font size="5" face="Century"> Project Description </font> </h4> | ||
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<p> This year, the theme of iGEM Gifu is to synthesize a long chain protein with circular mRNA. It is a sequel to our theme of the last year.</p><br> | <p> This year, the theme of iGEM Gifu is to synthesize a long chain protein with circular mRNA. It is a sequel to our theme of the last year.</p><br> | ||
Here, we explain our theme of the last year. | Here, we explain our theme of the last year. | ||
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<p> We succeeded in expression of the circular mRNA, and synthesizing long chain protein last year. But the long chain protein synthesized in last year was not functional because its holding had lost shape. So, this year, we design a linker sequence and synthesize a functional long chain protein. On the other hand, the probability was just a few percent that the cyclization happens. It is thought that the ribozyme site of the splicing is far from the reactive site, and it lowers the probability. So we’ll design complemental sequence near the ribozyme site and the reactive site of splicing to make them accessible to each other, and raise the probability of the cyclization in the theme.</p> | <p> We succeeded in expression of the circular mRNA, and synthesizing long chain protein last year. But the long chain protein synthesized in last year was not functional because its holding had lost shape. So, this year, we design a linker sequence and synthesize a functional long chain protein. On the other hand, the probability was just a few percent that the cyclization happens. It is thought that the ribozyme site of the splicing is far from the reactive site, and it lowers the probability. So we’ll design complemental sequence near the ribozyme site and the reactive site of splicing to make them accessible to each other, and raise the probability of the cyclization in the theme.</p> | ||
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Revision as of 04:58, 24 July 2015