Latest revision as of 09:47, 27 July 2015

Friday 17th July

Lab Work

Ligation

by Pauline

In each tube:

3 µL Buffer DNA pol T4
1µL DNA pol T4
4µL H2O

BBa_J23101 (10µL) + BBa_S03518 (12µL)
BBa_S03518 (12µL) + BBa_B0015 (10µL)
BBa_B0015 (10µL) + BBa_I13602 (12µL)

Incubation 4h, 4°C

New culture

by Coralie

1696: Bad::Tet
1320:ClpP::Cm
1693:z1:Spect

On LB plates, incubation ON, 37°C

Transformation

by Johan

Transformation with ligation product:

BBa_J23101 + BBa_S03518 = BBa_K1707001
BBa_B0015 + BBa_I13602 = BBa_K1707002
BBa_S03518 + BBa_B0015 = BBa_K1707003

New culture on plates

by Pauline

BBa_J23101 + BBa_I13504
BBa_J23106 + BBa_I13504
BBa_J23117 + BBa_I13504
BBa_B0030
BBa_R0051

Plasmid extraction

by Pauline and Seong Koo

BBa_K1707000
BBa_K1399019
BBa_K1399005
BBa_K1399023

Cultures from the 16/15/2015 With the Nucleospin Kit from Macherey Nagel

Members present:

Instructors and advisors: Alice.
Students: Johan, Seong Koo, Coralie, Pauline

Back to the calendar

@@ Line 1: / Line 1: @@
+{{Team:Paris_Saclay/notebook_header}}
 =Friday 17th July=
+==Lab Work==
+===Ligation===
-====Ligation ====
 ''by Pauline''
@@ Line 10: / Line 10: @@
 * 4µL H2O
-Biobricks:
+* BBa_J23101 (10µL) + BBa_S03518 (12µL)
-* J23101 (10µL) + S03518 (12µL)
+* BBa_S03518 (12µL) + BBa_B0015 (10µL)
-* S03518 (12µL) + B0015 (10µL)
+* BBa_B0015 (10µL) + BBa_I13602 (12µL)
-* B0015 (10µL) + I13602 (12µL)
 Incubation 4h, 4°C
+===New culture===
-====New culture====
 ''by Coralie''
@@ Line 28: / Line 25: @@
 On LB plates, incubation ON, 37°C
+===Transformation===
-====Transformation====
 ''by Johan''
 Transformation with ligation product:
-* J23101 + S03518 = K1707001
+* BBa_J23101 + BBa_S03518 = BBa_K1707001
-* S03518 + B0015 = K1707003
+* BBa_B0015 + BBa_I13602 = BBa_K1707002
-* B0015 + I13602 = K1707002
+* BBa_S03518 + BBa_B0015 = BBa_K1707003
+===New culture on plates===
-====New culture on plates====
 ''by Pauline''
-* J23101 + I13504
+* BBa_J23101 + BBa_I13504
-* J23106 + I13504
+* BBa_J23106 + BBa_I13504
-* J23117 + I13504
+* BBa_J23117 + BBa_I13504
-* B0030
+* BBa_B0030
-* R0051
+* BBa_R0051
+===Plasmid extraction===
+''by Pauline and Seong Koo''
-=====Plasmid extraction =====
+* BBa_K1707000
-''by Pauline and Seong-Koo''
+* BBa_K1399019
+* BBa_K1399005
-Biobricks:
+* BBa_K1399023
-* K1707000
-* K1399019
-* K1399005
-* K1399023
 Cultures from the 16/15/2015
 With the Nucleospin Kit from Macherey Nagel
 '''Members present:'''
 * Instructors and advisors: Alice.
 * Students: Johan, Seong Koo, Coralie, Pauline
+{{Team:Paris_Saclay/notebook_footer}}

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