Difference between revisions of "Team:KU Leuven/Wetlab/Background"
| (4 intermediate revisions by the same user not shown) | |||
| Line 52: | Line 52: | ||
<div class="summarytext1"> | <div class="summarytext1"> | ||
<div class="part"> | <div class="part"> | ||
| − | + | <p><h2>Lexicon</h2> | |
| + | Temperature sensitive cI_857= Mutated variant of the phage lambda repressor protein. It can bind firmly to the lambda promotor and represses transcription at low temperatures, but is unstable at elevated temperatures. | ||
| + | </br> | ||
| + | LuxI = enzyme for production an AHL called oxohexanoyl-homoserine lactone. This highly diffusible molecule is used as a communication molecule between cells, and is able to bind with LuxR at the lux promotor to initiate transcription. | ||
| + | </br> | ||
| + | LuxR = cytoplasmic receptor for AHL | ||
| + | </br> | ||
| + | Ag43 = membrane protein, causes autoaggregation of cells | ||
| + | </br> | ||
| + | IlvE = last enzyme in the biosynthesis of leucine. In order for it to work, we need to provide the right substrate:4-methyl-2-oxopentanoate to the medium. | ||
| + | </br> | ||
| + | Leucine = amino acid, but also a repellent if tar is knocked out | ||
| + | </br> | ||
| + | GFP = green fluorescent protein | ||
| + | </br> | ||
| + | YFP = yellow fluorescent protein | ||
| + | </br> | ||
| + | RFP = red fluorescent protein | ||
| + | </br> | ||
| + | PenI = Repressor protein, binds to the PenI regulated promotor | ||
| + | </br> | ||
| + | LVA = degradation tag, signals the linked protein for intracellular degradation | ||
| + | </br> | ||
| + | His-tag: repeat of six histidines, in order to purify it with nickelbeads | ||
| + | </br> | ||
| + | E-tag: links the tagged protein with an anti-E antibody | ||
| + | </br> | ||
| + | <br/> | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 94: | Line 122: | ||
<div class="subimg"> | <div class="subimg"> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/d/dd/InternalCover.png" width="100%"> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | |
</div> | </div> | ||
| Line 112: | Line 129: | ||
<div class="subimg"> | <div class="subimg"> | ||
| − | <img src="https://static.igem.org/mediawiki/2015/ | + | <a href="https://2015.igem.org/Team:KU_Leuven/Wetlab"> |
| + | <!---<img src="https://static.igem.org/mediawiki/2015/2/2b/KU_Leuven_Monocotyl.jpg" width="100%" height="100%" >--> | ||
| + | </a> | ||
</div> | </div> | ||
| − | |||
| − | |||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
<div class="subtext"> | <div class="subtext"> | ||
<h2>Parts</h2> | <h2>Parts</h2> | ||
| − | <p> | + | <p> |
| − | + | Coming Soon | |
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 134: | Line 147: | ||
<div class="subtext"> | <div class="subtext"> | ||
<h2>Methods</h2> | <h2>Methods</h2> | ||
| − | <p> | + | <p> |
| − | + | Coming Soon | |
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 145: | Line 157: | ||
<div class="subtext"> | <div class="subtext"> | ||
<h2>Results</h2> | <h2>Results</h2> | ||
| − | <p> | + | <p> |
| − | + | Coming Soon | |
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
| − | < | + | <div class="whitespace"> |
</div> | </div> | ||
<div class="subtext"> | <div class="subtext"> | ||
| − | <a href="https://2015.igem.org/Team:KU_Leuven/ | + | <a href="https://2015.igem.org/Team:KU_Leuven/Wetlab"> |
<h2>Back</h2> | <h2>Back</h2> | ||
</a> | </a> | ||
Latest revision as of 07:10, 29 July 2015
1-D continuous model
Lexicon
Temperature sensitive cI_857= Mutated variant of the phage lambda repressor protein. It can bind firmly to the lambda promotor and represses transcription at low temperatures, but is unstable at elevated temperatures. LuxI = enzyme for production an AHL called oxohexanoyl-homoserine lactone. This highly diffusible molecule is used as a communication molecule between cells, and is able to bind with LuxR at the lux promotor to initiate transcription. LuxR = cytoplasmic receptor for AHL Ag43 = membrane protein, causes autoaggregation of cells IlvE = last enzyme in the biosynthesis of leucine. In order for it to work, we need to provide the right substrate:4-methyl-2-oxopentanoate to the medium. Leucine = amino acid, but also a repellent if tar is knocked out GFP = green fluorescent protein YFP = yellow fluorescent protein RFP = red fluorescent protein PenI = Repressor protein, binds to the PenI regulated promotor LVA = degradation tag, signals the linked protein for intracellular degradation His-tag: repeat of six histidines, in order to purify it with nickelbeads E-tag: links the tagged protein with an anti-E antibody
References
