Difference between revisions of "Team:Glasgow/Notebook"
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| + | <p>The 4 strains of E. coli used are: MGI655ZI (which has tetR, and lacIq, DS941 (recF-), TOP10 (recA-), DH5α (recA-). We designed Phlf and SrpR promoters as BioBricks to be ordered from IDT as if their prefix and suffix were cut with EcoRI and SpeI.</p> | ||
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| − | <span class="date"> | + | <span class="date">15th</span> |
<span class="month">Jun</span> | <span class="month">Jun</span> | ||
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| − | <p></p> | + | <p> We grew bacteria from a single colony from an agar plate in liquid culture to prepare for the dilution series. We designed the Phlf and SrpR respressor sequences with a prefix, B0032 ribosome binding site, TACTAG scar sequence, the repressor itself, TACTAGAG scar sequence, B0010 extended terminator with the help of Steve, and suffix.<br> |
| + | The biggest concern of Marshal and John was that it would be difficult to sell the idea (it is hard to convince the audience there is an actual demand and advantage to our idea since it’s basically a massive release of GMO). | ||
| + | An advantage is that if we make it work, we have a modular design. | ||
| + | To test a terminator we need a fluorescent protein either after the terminator or before it. | ||
| + | </p> | ||
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| − | <span class="date"> | + | <span class="date">16th</span> |
<span class="month">Jun</span> | <span class="month">Jun</span> | ||
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Revision as of 13:36, 29 July 2015
We went to Dundee University to attend the Scotland IGEM team's meeting. We had so much fun in the meeting, sharing different ideas with each team.
The 4 strains of E. coli used are: MGI655ZI (which has tetR, and lacIq, DS941 (recF-), TOP10 (recA-), DH5α (recA-). We designed Phlf and SrpR promoters as BioBricks to be ordered from IDT as if their prefix and suffix were cut with EcoRI and SpeI.
We grew bacteria from a single colony from an agar plate in liquid culture to prepare for the dilution series. We designed the Phlf and SrpR respressor sequences with a prefix, B0032 ribosome binding site, TACTAG scar sequence, the repressor itself, TACTAGAG scar sequence, B0010 extended terminator with the help of Steve, and suffix.
The biggest concern of Marshal and John was that it would be difficult to sell the idea (it is hard to convince the audience there is an actual demand and advantage to our idea since it’s basically a massive release of GMO).
An advantage is that if we make it work, we have a modular design.
To test a terminator we need a fluorescent protein either after the terminator or before it.