Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/28 July 2015"

(Created page with "{{Template:UCLA}} =7/28/2015= ==Miniprep and Sequencing== *Miniprep (qiagen) for M2-12(1C3) liquid culture. *Sequencing with VF, VR. ==Maxiprep for M2-AB, BC, CA== *Maxiprep u...")
 
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[[File:7 28 2015 UCLA ICA.jpg|none|thumb|500px|'''Fig. 1''' BsaI Digestion of M1-AB, BC, CA. The expected product size is 102 bp.]]
 
[[File:7 28 2015 UCLA ICA.jpg|none|thumb|500px|'''Fig. 1''' BsaI Digestion of M1-AB, BC, CA. The expected product size is 102 bp.]]
 
*Excised the 102 bp bands for gel extraction
 
*Excised the 102 bp bands for gel extraction
 +
*Also saved some of the digested backbone as linearized pSB1C3.

Latest revision as of 22:46, 30 July 2015

iGEM UCLA




7/28/2015

Miniprep and Sequencing

  • Miniprep (qiagen) for M2-12(1C3) liquid culture.
  • Sequencing with VF, VR.

Maxiprep for M2-AB, BC, CA

  • Maxiprep using qiagen kit.
    • M1-AB: 111 ng/uL
    • M1-BC: 150 ng/uL
    • M1-CA: 350 ng/uL

BsaI Digestion for M1-AB, BC, CA

  • Olivia digested 5 ug of relevant plasmid in a 50 uL reaction with 4 uL of BsaI.
    • Digest 50 C 2 hrs, heat kill 65 C 20 min.
  • Run on 1.5% TAE gel to visualize. Used 3 uL of NEB 100 bp ladder.
Fig. 1 BsaI Digestion of M1-AB, BC, CA. The expected product size is 102 bp.
  • Excised the 102 bp bands for gel extraction
  • Also saved some of the digested backbone as linearized pSB1C3.