Difference between revisions of "Team:KU Leuven/Wetlab"

 
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<h2>Wet Lab</h2>
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<h2>Research</h2>
 
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Patterns are fascinating, from the veins of a leaf to the spots on a zebra, from a single cell to a whole organism. Patterns are found everywhere in nature, but how these are formed is not entirely clear. We, the KU Leuven 2015 iGEM team, decided to work on the fundamental mechanisms behind pattern formation. The way cells interact to generate a specific pattern has triggered our curiosity and added a new dimension to the way the patterns are looked upon. Our mission is to create different and astonishing biological patterns with engineered bacteria for a better understanding of nature with the prospect of applying the knowledge in industry.
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We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.  
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
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  <h2>Plasmids</h2>
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  <h2>Background</h2>
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  <p>“ We are not gonna buy that; We can make it “
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  <p> A detailed description about the interaction between our two cells and the genetic circuit can be found here.
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When designing plasmids, we need to add tags and fluorescent proteins to quantify our parameters. We will also add extra restriction sites to easily generate biobricks.
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<a href="https://2015.igem.org/Team:KU_Leuven/Wetlab/About">Read more</a>
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<a href="https://2015.igem.org/Team:KU_Leuven/Wetlab/Background">Read more</a>
 
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  <h2>Experiments</h2>
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  <p>Here you can find the following steps to become our modified organism and detailed quantification methods to determine interesting parameters.
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<p>A list of all used biobricks, the modifications we performed on them and the new ones we designed.
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
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  <p>Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
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  <p>The results of our experiments will appear here.  
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  <p>The results of our experiments will appear here.
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
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Latest revision as of 14:47, 31 July 2015

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We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.


Background

A detailed description about the interaction between our two cells and the genetic circuit can be found here.
Read more

Parts

A list of all used biobricks, the modifications we performed on them and the new ones we designed.
Read more

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters
Read more

Results

The results of our experiments will appear here.
Read more