Difference between revisions of "Team:KU Leuven/Wetlab"

 
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We designed a circuit capable of producing patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger this structure formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns, providing advancements in a variety of industrial processes like for example the creation of novel bio-materials. On the other hand, this fundamental project could also speed up medical research projects on among others tumor formation and tissue regeneration.
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We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.  
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  <h2>Project</h2>
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  <h2>Background</h2>
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  <p>We decided to work on the fundamental mechanisms behind pattern formation. We design two bacteria strains which interact with each other. To achieve our goal, the link between wet lab and modeling will be crucial to the successful design of our experiments.
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  <p> A detailed description about the interaction between our two cells and the genetic circuit can be found here.
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
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<a href="https://2015.igem.org/Team:KU_Leuven/Wetlab/Background">Read more</a>
 
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  <h2>Wet lab</h2>
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  <h2>Parts</h2>
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  <p>Swift and smart in actions to design the plasmids, quantify the parameters and to create the patterns. Join us in our exciting journey.
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  <p>A list of all used biobricks, the modifications we performed on them and the new ones we designed.
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
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  <h2>Modeling</h2>
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  <h2>Methods</h2>
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  <p>The modeling team fit models to the data obtained by the wet lab. Continuous and hybrid models will be used. Hybrid models are models which have a discrete and continuous part. Synergically, simulations from the cyber lab will aid tuning the experimental conditions that lead to the desirable patterns.
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  <p>Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
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  <h2>Team</h2>
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  <h2>Results</h2>
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  <p>Diverse with respect to thoughts, perspectives, nationalities and languages, yet bound together by our enthusiasm for science and research. Would you like to know more about us?
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  <p>The results of our experiments will appear here.
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
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<h2>Outreach</h2>
 
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<p>Here comes everything about education and sponsors.
 
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<a href="https://2015.igem.org/Team:KU_Leuven/Project/About">Read more</a>
 
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Latest revision as of 14:47, 31 July 2015

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We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.


Background

A detailed description about the interaction between our two cells and the genetic circuit can be found here.
Read more

Parts

A list of all used biobricks, the modifications we performed on them and the new ones we designed.
Read more

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters
Read more

Results

The results of our experiments will appear here.
Read more