Difference between revisions of "Team:Aalto-Helsinki/LabBook"

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<table >
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  <tr>
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    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td>
 +
    <td class="bottomline"><h3>Things done</h3></td>
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    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
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    <td class="bottomline"></td>
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    <td class="bottomline"></td>
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    <td class="bottomline"></td>
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    <td class="bottomline"><h3>May 18th - May 22nd<br>Monday</h3></td>
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  </tr>
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  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Propagations</h3></div></td>
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        <td class="white arrow" >
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            <p style="font-size:12px">Transformed <br/>AH007-AH009,<br/> AH005 & AH019 <br/> into TOP10</p>
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        </td>
 +
        <td class="blue arrow" >
 +
            <p>AH007-AH009 <br/>& AH019</p>
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        </td>
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        <td class="white arrow" >
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            <p>o/n cultures</p>
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        </td>
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        <td class="blue arrow" >
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            <p>MiniPrep</p>
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        </td>
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  </tr>
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  <tr>
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<td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
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        <td>
 +
        </td>
 +
 +
        <td class="red arrow" >
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            <p>No colonies <br/> for AH005</p>
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        </td>
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        <td class="white arrow" >
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            <p>Re-transform <br/> into TOP10</p>
 +
        </td>
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        <td class="white arrow" >
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            <p>Plates in rt <br/> over weekend</p>
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        </td>
 +
  </tr>
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  <tr>
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        <td class="first rightline"><div class="heightdiv"><h3>Propagations</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>o/n culture of<br/>AH001-AH004 <br/> & AH006</p>
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        </td>
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        <td class="blue arrow" >
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            <p >MiniPrep</p>
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        </td>
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  </tr>
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  <tr>
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        <td class="first rightline"><div class="heightdiv"><h3>Competent Cells</h3></div></td>
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        <td class="white arrow" >
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            <p>o/n culture <br/> of BL21(DE3) <br/> transformants</p>
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        </td>
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        <td class="blue arrow" >
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            <p >MiniPrep</p>
 +
        </td>
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        <td class="red arrow" >
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            <p>PCR with <br/>VF2 & VR</p>
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        </td>
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        <td class="red arrow" >
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            <p>Smeared gels</p>
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        </td>
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  </tr>
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    <tr>
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        <td class="first rightline"><div class="heightdiv"><h3>Assembly</h3></div></td>
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        <td class="white arrow" >
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            <p>Restrict <br/>AH001-AH004 <br/> & AH006-AH008</p>
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        </td>       
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        <td class="white arrow" >
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            <p>Ligation</p>
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        </td>       
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        </td>       
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        <td class="white arrow" >
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            <p>Stored in <br/>+4°C</p>
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        </td>       
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</tr>
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<tr>
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        <td class="first rightline"><div class="heightdiv"><h3></h3></div></td>
 +
       
 +
        <td>
 +
           
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>No colonies for <br/>&nbsp;&nbsp; AH005, AH007 <br/> or AH008</p>
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        </td>
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  </tr>
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 +
</table>
  
 
<h2 id="week3">Week 3</h2>
 
<h2 id="week3">Week 3</h2>

Revision as of 12:34, 3 August 2015

Lab Book

An example of what the table could look like:

Construct
worked with

Things done

Lab 1.1.2015
Monday

CAR

Here is my text

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text, there
is lot of it

another CAR?

Here is my text

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Here is my
text, there
is lot of it

Ado?

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Ado cont.

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Here is my text

Here is my
text, there
is lot of it

Week 1

Construct
worked with

Things done

May 18th - May 22nd
Monday

Plates

Making Cm
plates

Stored in
+4°C

Competent Cells

Preparing
solutions for
competent cells

Stored in
+4°C

Competent Cells

o/n culture
of E. coli
TOP10

Chemical
competent
cells protocol

Tranformation
efficiency kit

Competent Cells

o/n culture
of E. coli
BL21(DE3)

Chemical
competent
cells protocol

Tranformation
efficiency kit

Colonies are
the wrong color

Propagations

Transformed
AH001-AH008
into TOP10

AH001-AH004
& AH006

Stored in
+4°C

No colonies for
   AH005, AH007
or AH008

Week 2

Construct
worked with

Things done

May 18th - May 22nd
Monday

Propagations

Transformed
AH007-AH009,
AH005 & AH019
into TOP10

AH007-AH009
& AH019

o/n cultures

MiniPrep

No colonies
for AH005

Re-transform
into TOP10

Plates in rt
over weekend

Propagations

o/n culture of
AH001-AH004
& AH006

MiniPrep

Competent Cells

o/n culture
of BL21(DE3)
transformants

MiniPrep

PCR with
VF2 & VR

Smeared gels

Assembly

Restrict
AH001-AH004
& AH006-AH008

Ligation

Stored in
+4°C

No colonies for
   AH005, AH007
or AH008

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 16

Week 17

Week 18