Difference between revisions of "Team:Aalto-Helsinki/LabBook"

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<h2 id="week3">Week 3</h2>
 
<h2 id="week3">Week 3</h2>
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<table >
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  <tr>
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    <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td>
 +
    <td class="bottomline"><h3>Things done</h3></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
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    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"></td>
 +
    <td class="bottomline"><h3>June 1st - June 5th</h3></td>
 +
  </tr>
 +
  <tr>
 +
        <td class="first rightline"><div class="heightdiv"><h3>Assembly <br/> Testing RBS's</h3></div></td>
 +
 +
        <td class="red arrow" >
 +
            <p>No colonies <br/>for AH011 <br/>or AH014</p>
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        </td>
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        <td class="white arrow" >
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            <p>re-assemble</p>
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        </td>
 +
        <td class="blue arrow" >
 +
            <p>Restrict & <br/> extract <br/> from gel</p>
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        </td>
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        <td class="white arrow" >
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            <p>Ligate & <br/> tranform <br/> into TOP10</p>
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        </td>
 +
        </td>
 +
        <td class="red arrow" >
 +
            <p>No colonies</p>
 +
        </td>
 +
  </tr>
 +
  <tr>
 +
<td class="first rightline"><div class="heightdiv"><h3>Assembly <br/> Testing RBS's</h3></div></td>
 +
 +
        <td class="blue arrow" >
 +
            <p>AH005 &<br/> AH013 OK</p>
 +
        </td>
 +
        <td class="white arrow" >
 +
            <p>o/n cultures</p>
 +
        </td>
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        <td class="blue arrow" >
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            <p>MiniPrep</p>
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        </td>
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  </tr>
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  <tr>
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        <td class="first rightline"><div class="heightdiv"><h3>Propagations</h3></div></td>
 +
        <td class="white arrow" >
 +
            <p>Tranform <br/>&nbsp;&nbsp;&nbsp;&nbsp; AH020 & AH021 <br/> into TOP10</p>
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        </td>
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        <td class="white arrow" >
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            <p>Plates in rt <br/> over weekend</p>
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        </td>
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  </tr>
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 +
 +
</table>
 +
 
<h2 id="week4">Week 4</h2>
 
<h2 id="week4">Week 4</h2>
 
<h2 id="week5">Week 5</h2>
 
<h2 id="week5">Week 5</h2>

Revision as of 07:51, 4 August 2015

Lab Book

An example of what the table could look like:

Construct
worked with

Things done

Lab 1.1.2015
Monday

CAR

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text, there
is lot of it

another CAR?

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Here is my
text, there
is lot of it

Ado?

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Ado cont.

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Here is my text

Here is my text

Here is my text

Here is my
text, there
is lot of it

Week 1

Construct
worked with

Things done

May 18th - May 22nd

Plates

Making Cm
plates

Stored in
+4°C

Competent Cells

Preparing
solutions for
competent cells

Stored in
+4°C

Competent Cells

o/n culture
of E. coli
TOP10

Chemical
competent
cells protocol

Tranformation
efficiency kit

Competent Cells

o/n culture
of E. coli
BL21(DE3)

Chemical
competent
cells protocol

Tranformation
efficiency kit

Colonies are
the wrong color

Propagations

Transformed
AH001-AH008
into TOP10

AH001-AH004
& AH006

Stored in
+4°C

No colonies for
   AH005, AH007
or AH008

Week 2

Construct
worked with

Things done

May 25th - May 29th

Propagations

Transformed
AH007-AH009,
AH005 & AH019
into TOP10

AH007-AH009
& AH019

o/n cultures

MiniPrep

No colonies
for AH005

Re-transform
into TOP10

Plates in rt
over weekend

Propagations

o/n culture of
AH001-AH004
& AH006

MiniPrep

Competent Cells

o/n culture
of BL21(DE3)
transformants

MiniPrep

PCR with
VF2 & VR

Smeared gels

Assembly
Testing RBS's

Restrict
AH001-AH004
& AH006-AH008

Ligation

AH002+
  AH004+AH008
-> AH014

Repeat
ligation

Restrict &
extract
from gel

Transformation
into TOP10

Plates in rt
over weekend

AH001+
   AH006+AH008
-> AH011

Repeat
ligation

Restrict &
extract
from gel

Transformation
into TOP10

Plates in rt
over weekend

AH001+
   AH003+AH008
-> AH013

Repeat
ligation

Restrict &
extract
from gel

Transformation
into TOP10

Plates in rt
over weekend

Week 3

Construct
worked with

Things done

June 1st - June 5th

Assembly
Testing RBS's

No colonies
for AH011
or AH014

re-assemble

Restrict &
extract
from gel

Ligate &
tranform
into TOP10

No colonies

Assembly
Testing RBS's

AH005 &
AH013 OK

o/n cultures

MiniPrep

Propagations

Tranform
     AH020 & AH021
into TOP10

Plates in rt
over weekend

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 16

Week 17

Week 18