Difference between revisions of "Team:UiOslo Norway"

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<h1> Methane Incorporated </h1>
 
<h1> Methane Incorporated </h1>
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<p><h5>Methane  (CH<sub>4</sub>)  is  the  second  most  prevalent  emitted  greenhouse  gas  on  earth.  
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<p><h5><font color="black">
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Methane  (CH<sub>4</sub>)  is  the  second  most  prevalent  emitted  greenhouse  gas  on  earth.  
 
Today we are facing the challenge of solving this problem of high methane concentrations in the atmosphere that leads to global warming.  The  global  warming  potential  of  methane  is  about  84  
 
Today we are facing the challenge of solving this problem of high methane concentrations in the atmosphere that leads to global warming.  The  global  warming  potential  of  methane  is  about  84  
 
times higher than that of carbon dioxide (CO<sub>2</sub>) when compared over 20 years.   
 
times higher than that of carbon dioxide (CO<sub>2</sub>) when compared over 20 years.   
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Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used. </h5></p>
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Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used.  
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</font></h5></p>
  
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<p><h5>The goal of our  project is  to  develop an <i>Escherichia coli</i> (<i>E. coli</i>)
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<p><h5><font color="black">
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The goal of our  project is  to  develop an <i>Escherichia coli</i> (<i>E. coli</i>)
 
based  model  that filters methane out of the air and converts this into biomass.  First part of the project is to break down methane to methanol with the enzyme complex soluble  methane  monooxygenase (sMMO)  of  the  
 
based  model  that filters methane out of the air and converts this into biomass.  First part of the project is to break down methane to methanol with the enzyme complex soluble  methane  monooxygenase (sMMO)  of  the  
 
methanotroph <i>Methylococcus  capsulatus</i> expressed in <i>E.  coli</i>.  In  order  for  the  multisubunit  
 
methanotroph <i>Methylococcus  capsulatus</i> expressed in <i>E.  coli</i>.  In  order  for  the  multisubunit  
 
enzyme complex to break down methane to methanol, oxygen and iron-ions are required. Second  
 
enzyme complex to break down methane to methanol, oxygen and iron-ions are required. Second  
 
part of the project is to convert methanol into biomass using the Ribulose-Monophosphate (RuMP)- pathway and establish this pathway in  <i>E.coli.</i>  The  pathway  is  found  in  the  methanotroph <i>Bacillus  methanolicus</i> and enables  
 
part of the project is to convert methanol into biomass using the Ribulose-Monophosphate (RuMP)- pathway and establish this pathway in  <i>E.coli.</i>  The  pathway  is  found  in  the  methanotroph <i>Bacillus  methanolicus</i> and enables  
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<i>E. coli</i> to convert methanol into biomass.</h5></p>
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<i>E. coli</i> to convert methanol into biomass.
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</font></h5></p>
  
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<p><h5>To test the functionality of the modified <i>E. coli</i>, the bacteria will be grown in a closed  
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<p><h5><font color="black">
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To test the functionality of the modified <i>E. coli</i>, the bacteria will be grown in a closed  
 
system  under  methane  rich  conditions.  Additionally, a  filter  will be  created  that  can  
 
system  under  methane  rich  conditions.  Additionally, a  filter  will be  created  that  can  
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contain the modified <i>E.coli</i> and can filter the surrounding air through the system, so that the methane can be broken down. The filter could be used in areas where methane emission is a problem. </h5>  </p>
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contain the modified <i>E.coli</i> and can filter the surrounding air through the system, so that the methane can be broken down. The filter could be used in areas where methane emission is a problem.  
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</font></h5>  </p>
  
  

Revision as of 10:47, 4 August 2015

Methane Incorporated

Methane (CH4) is the second most prevalent emitted greenhouse gas on earth. Today we are facing the challenge of solving this problem of high methane concentrations in the atmosphere that leads to global warming. The global warming potential of methane is about 84 times higher than that of carbon dioxide (CO2) when compared over 20 years. Technical methods to reuse emitted methane gas are cost- and time- intensive and therefore rarely used.

The goal of our project is to develop an Escherichia coli (E. coli) based model that filters methane out of the air and converts this into biomass. First part of the project is to break down methane to methanol with the enzyme complex soluble methane monooxygenase (sMMO) of the methanotroph Methylococcus capsulatus expressed in E. coli. In order for the multisubunit enzyme complex to break down methane to methanol, oxygen and iron-ions are required. Second part of the project is to convert methanol into biomass using the Ribulose-Monophosphate (RuMP)- pathway and establish this pathway in E.coli. The pathway is found in the methanotroph Bacillus methanolicus and enables E. coli to convert methanol into biomass.

To test the functionality of the modified E. coli, the bacteria will be grown in a closed system under methane rich conditions. Additionally, a filter will be created that can contain the modified E.coli and can filter the surrounding air through the system, so that the methane can be broken down. The filter could be used in areas where methane emission is a problem.