Difference between revisions of "Team:Warwick/Modelling4"

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<h5 class="sidebartitle">DNA Beading</h5>
 
<h5 class="sidebartitle">DNA Beading</h5>
 
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<img src="https://static.igem.org/mediawiki/2015/b/ba/WarwickE.coli_Beads.png" class="pics" alt=""> This shows how the DNA strands come together. Three double stranded strings of DNA are denatured and then when slowly cooled will come together to form the Y shape. However after the denaturing each strand of DNA has an equal chance of bonding to the original piece of DNA as it does to the correct origami side. Therefore the more complex the structure the less likely it is that that structure will fully form.  
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<img src="https://static.igem.org/mediawiki/2015/b/ba/WarwickE.coli_Beads.png" class="pics" alt="">  
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One option we came up with is to create a string of DNA which has Zinc finger binding sites at certain spots so that you could create a pattern of different E.coli cells which could be used to study microbial communities.
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This could potentially then be used to create 2D shapes and images, by combing strings of DNA with the bonded E.coli cells along them to create something like the image below.
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To make the DNA beads we will use an E.coli genome and then denature the double stranded DNA and then add in primers at different locations with the zinc finger binding sequence attached to the end.
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Revision as of 11:40, 6 August 2015

Warwick iGEM 2015

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DNA Beading

Minimum E.coli for Image

The image on the left shows the minimum number of cells needed to produce a clear image with a discernible shape. For this we looked at simple shapes to see how complexity increased the number of cells or pixels needed.
The image on the right shows the increase of E.coli cells needed to make a shape. The the number of cells per shape follows a linear progression, proportional to the number of sides that the shape has. A basic first order, linear differntial equation would be C=13(S-2)+12, where C is the approximate number of cells needed to make that shape and S is the number of sides of the shape.

DNA Beading

One option we came up with is to create a string of DNA which has Zinc finger binding sites at certain spots so that you could create a pattern of different E.coli cells which could be used to study microbial communities.
This could potentially then be used to create 2D shapes and images, by combing strings of DNA with the bonded E.coli cells along them to create something like the image below.
To make the DNA beads we will use an E.coli genome and then denature the double stranded DNA and then add in primers at different locations with the zinc finger binding sequence attached to the end.

Primer Sequences for Beads

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