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Revision as of 01:53, 7 August 2015

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home project measurement modeling human practice notebook team
Abstract
Gene regulation has typically employed chemically mediated expression systems, which are slow and could be limited by medium during drug diffusion. The development of remote regulation systems that could response to physical signals is in demand. This summer, we are trying to design a platform responsed to electromagnetic signal. This platform is composed of 2 sessions : magnetic receiver and thermosensitive regulator.
Receiver
For magnetic receiver, we chose ferritin, the iron-storage protein in organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core would be heated, which would trigger thermosensitive regulator.
Regulator
For thermosensitive regulator, we chose RNA thermometer and constructed a thermosensitive T7 RNA polymerase. RNA thermometer is a structured RNA which could expose RBS only when heated. The thermosensitive T7 RNA polymerase is a normal T7 RNA polymerase at the selected locus of which was inserted by a temperature-sensitive intein, which could self-splice at specific temperature.
Achievement
123
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