Difference between revisions of "Team:NCTU Formosa/Practices"

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<h2> Human Practices </h2>
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        <ul style="list-style-image: none; color: #fff;">
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            <li class="navli" style="background-color:#ffffff"><navtext>Project</navtext></li>
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            <li class="navli"><navtext>Background</navtext></li>
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            <li class="navli"><navtext>Design</navtext></li>
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            <li class="navli"><navtext>Result</navtext></li>
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            <li class="navli"><navtext>Modeling</navtext></li>
  
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<p>iGEM teams are unique and leading the field because they "go beyond the lab" to imagine their projects in a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
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        </ul>
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<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
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<p>For more information, please see the <a href="https://2015.igem.org/Practices_Hub">Practices Hub</a>.</p>
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    <div class="p01"><g1>PROJECT</g1></div>
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    <div class="upper"><g1>Background / </g1><g2>Our project focuses on the diagnoses before and after treatment for cancers.</g2>
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<h4>Note</h4>
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        </div>
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<p>You must fill out this page in order to be considered for all <a href="https://2015.igem.org/Judging/Awards">awards</a> for Human Practices:</p>
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        <div class="side">
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<ul>
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<li>Human Practices silver medal criterion</li>
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        <div class="textbox">Our project focuses on the diagnoses before and after treatment for cancers. Two systems are constructed for our project. The first system displays scFv on the surface of E.coli while the second one displays gold binding peptide (GBP). 
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<li>Human Practices gold medal criterion</li>
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Before treatment, by using the fluorescent E.coli with scFv displayed on the surface, IHC procedure can be simplified because secondary antibodies is omitted and solves the problem of non-specific binding of secondary antibodies.
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<li>Best Integrated Human Practices award</li>
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  A dual system involving E.coli displaying scFv and GBP can solve random orientation of antibodies coating on gold chip to attain an accurate measurement. Therefore, the dual system can also offer a quantitative and accurate diagnosis for after treatment of cancer.  
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<li>Best Education and Public Engagement award</li>
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Our project not only improves the diagnoses of the cancer types before treatment easily but also helps track the patient's condition after treatment by multifaceted measurement technology with more accurate quantitative results!
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        <div class="upper"><g1>Design / </g1><g2>To display scfv on the E.coli's surface we used a transmembrance fusion protein.</g2>
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        <div class="textbox">ScFv is a single chain variable fragment the binding site for antigen. Comparing to the entire antibody, scFv is smaller for E.coli to display it.To display scfv on the E.coli’s surface we used a transmembrance fusion protein. This fusion protein is composed of Lipoprotein and a small part of outermembrane protein A (OmpA). Its C terminal will connect with the scFv and display it onto the membrane surface of the E.coli.
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            <div class="upper"><g1>Result / </g1><g2>Sucessfully present our E.cotector</g2>
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    <div class="upper"><g1>Modeling / </g1><g2>Find out the best condition of our E.cotector</g2>
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<h5>Some Human Practices topic areas </h5>
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<ul>
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<li>Philosophy</li>
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<li>Public Engagement / Dialogue</li>
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<li>Education</li>
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<li>Product Design</li>
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<li>Scale-Up and Deployment Issues</li>
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<li>Environmental Impact</li>
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<li>Ethics</li>
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<li>Safety</li>
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<li>Security</li>
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<li>Public Policy</li>
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<li>Law and Regulation</li>
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<li>Risk Assessment</li>
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<h5>What should we write about on this page?</h5>
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<p>On this page, you should write about the Human Practices topics you considered in your project, and document any special activities you did (such as visiting experts, talking to lawmakers, or doing public engagement).</p>
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<h5>Inspiration</h5>
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<p>Read what other teams have done:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:Dundee/policypractice/experts">2014 Dundee </a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Policy_Practices_Overview">2014 UC Davis </a></li>
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<li><a href="https://2013.igem.org/Team:Manchester/HumanPractices">2013 Manchester </a></li>
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<li><a href="https://2013.igem.org/Team:Cornell/outreach">2013 Cornell </a></li>
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</ul>
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<h3>Integrated Human Practices</h3>
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<p>Do you want to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Integrated Human Practices award</a>? Make it easy for the judges to find any wiki content that is relevant to this prize. Highlight this content with a header or separate section.</p>
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<h3>Education and Public Engagement</h3>
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<p>Do you want to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Education and Public Outreach award</a>? Make it easy for the judges to find any wiki content that is relevant to this prize. Highlight this content with a header or separate section.</p>
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Revision as of 04:08, 7 August 2015

  • Project
  • Background
  • Design
  • Result
  • Modeling
PROJECT
Background / Our project focuses on the diagnoses before and after treatment for cancers.
Our project focuses on the diagnoses before and after treatment for cancers. Two systems are constructed for our project. The first system displays scFv on the surface of E.coli while the second one displays gold binding peptide (GBP). Before treatment, by using the fluorescent E.coli with scFv displayed on the surface, IHC procedure can be simplified because secondary antibodies is omitted and solves the problem of non-specific binding of secondary antibodies. A dual system involving E.coli displaying scFv and GBP can solve random orientation of antibodies coating on gold chip to attain an accurate measurement. Therefore, the dual system can also offer a quantitative and accurate diagnosis for after treatment of cancer. Our project not only improves the diagnoses of the cancer types before treatment easily but also helps track the patient's condition after treatment by multifaceted measurement technology with more accurate quantitative results!
Design / To display scfv on the E.coli's surface we used a transmembrance fusion protein.
ScFv is a single chain variable fragment the binding site for antigen. Comparing to the entire antibody, scFv is smaller for E.coli to display it.To display scfv on the E.coli’s surface we used a transmembrance fusion protein. This fusion protein is composed of Lipoprotein and a small part of outermembrane protein A (OmpA). Its C terminal will connect with the scFv and display it onto the membrane surface of the E.coli.
Result / Sucessfully present our E.cotector
testestetsetsetset
Modeling / Find out the best condition of our E.cotector
testestetsetsetset