Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Competent E. coli 2"

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|content=<div class="text">A colony was picked from a plate (±14 days old, stored in fridge) and grown overnight in liquid media (5 mL LB in 50 mL tube) in shaker incubator at 37 °C, 200 rpm</div>
 
|content=<div class="text">A colony was picked from a plate (±14 days old, stored in fridge) and grown overnight in liquid media (5 mL LB in 50 mL tube) in shaker incubator at 37 °C, 200 rpm</div>
  
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{{Team:Groningen/TEMPLATES/LOG
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|title=00:00, 06 July 2015 - 00:00, 06 July 2015
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|executor=Harm Ruesink
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|content=<div class="text">
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Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).</div>
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<div class="listing">
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<div class="item"> 1µL of 50pg/µL BBa_J04450 </div>
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</div>
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<div class="text">
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Plate on LB agar + CM:
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</div>
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<div class="listing">
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<div class="item"> 100%  (100µL) </div>
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</div>
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<div class="text">
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Grow overnight in incubator at 37°C.</div>
 
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Revision as of 07:42, 7 August 2015

00:00, 06 July 2015 - 00:00, 06 July 2015
A colony was picked from a plate (±14 days old, stored in fridge) and grown overnight in liquid media (5 mL LB in 50 mL tube) in shaker incubator at 37 °C, 200 rpm
Harm Ruesink
00:00, 06 July 2015 - 00:00, 06 July 2015
Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).
1µL of 50pg/µL BBa_J04450

Plate on LB agar + CM:

100% (100µL)
Grow overnight in incubator at 37°C.
Harm Ruesink