Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Competent E. Coli 1"

 
(15 intermediate revisions by 2 users not shown)
Line 8: Line 8:
 
|title=00:00, 29 April 2015 - 00:00, 29 April 2015
 
|title=00:00, 29 April 2015 - 00:00, 29 April 2015
 
|executor=Harm
 
|executor=Harm
−
|content=<html> <div class="text"> Picked a colony from LB agar and grew overnight in liquid media (5mL LB in 50mL tube) in shaking incubator at 37°C, 220rpm. </div> </html>
+
|content=<html> <div class="text"> Picked a colony from LB agar and grew overnight in liquid media (5 mL LB in 50 mL tube) in shaking incubator at 37 °C, 220 rpm. </div> </html>
 
}}
 
}}
  
Line 14: Line 14:
 
|title=00:00, 30 April 2015 - 00:00, 30 April 2015
 
|title=00:00, 30 April 2015 - 00:00, 30 April 2015
 
|executor=Harm
 
|executor=Harm
−
|content=<html> <div class="text"> Transferred overnight culture in fresh LB (pre-warmed). 250µL culture in 25mL LB (1/100 dilution) and grew in shaker incubator at 37°C, 220rpm for 2h.
+
|content=<html>  
 +
 
 +
<div class="text"> Transferred overnight culture in fresh LB (pre-warmed). 250 µL culture in 25 mL LB (1/100 dilution) and grown in shaker incubator at 37 °C, 220 rpm for 2 hours.
 
</div>
 
</div>
−
<div class="text"
+
<div class="text">
−
Used culture (OD: 0,24) for making competent cells (protocol Clement) </div>
+
Used culture (OD: 0.24) for making competent cells (protocol Clement)  
−
<div class="test">
+
</div>
−
Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).
+
 
 +
<div class="text">
 +
 
 +
Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).</div>
 +
<div class="listing">
 +
<div class="item"> 1 µL of 50 pg/µL BBa_J04450 </div>
 +
</div>
 +
 
 +
<div class="text">
 +
Plate on LB agar + CM:
 +
</div>
 +
<div class="listing">
 +
<div class="item"> 10%  (10 µL) </div>
 +
<div class="item"> 90%  (90 µL) </div>
 +
<div class="item"> control </div>
 
</div>
 
</div>
 
</html>
 
</html>
 +
}}
 +
 +
{{Team:Groningen/TEMPLATES/LOG
 +
|title=00:00, 1 Juni 2015 - 00:00, 1 Juni 2015
 +
|executor=Harm
 +
|content=<html> <div class="text"> Checked transformation:
 +
A few red colonies on the 90% plate (± 22 colonies). </div> </html>
 
}}
 
}}

Latest revision as of 09:56, 7 August 2015

00:00, 28 April 2015 - 00:00, 28 April 2015

Plated E. coli DH5 alpha on LB agar and grew at 37°C for 24h.

Harm
00:00, 29 April 2015 - 00:00, 29 April 2015

Picked a colony from LB agar and grew overnight in liquid media (5 mL LB in 50 mL tube) in shaking incubator at 37 °C, 220 rpm.

Harm
00:00, 30 April 2015 - 00:00, 30 April 2015

Transferred overnight culture in fresh LB (pre-warmed). 250 µL culture in 25 mL LB (1/100 dilution) and grown in shaker incubator at 37 °C, 220 rpm for 2 hours.
Used culture (OD: 0.24) for making competent cells (protocol Clement)
Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).
1 µL of 50 pg/µL BBa_J04450
Plate on LB agar + CM:
10% (10 µL)
90% (90 µL)
control

Harm
00:00, 1 Juni 2015 - 00:00, 1 Juni 2015

Checked transformation: A few red colonies on the 90% plate (± 22 colonies).

Harm