Difference between revisions of "Team:Aalto-Helsinki/InterLabBook"
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m (Week 1 & 2) |
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<h2 id="week1">Week 1</h2> | <h2 id="week1">Week 1</h2> | ||
+ | <table > | ||
+ | <tr> | ||
+ | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td> | ||
+ | <td class="bottomline"><h3>Things done</h3></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"><h3>June 22nd - <br/> June 26th</h3></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Preparations</h3></div></td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Transform <br/> AHD1-AHD4 <br/>into TOP10</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>o/n culture</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>MinPrep</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Use iGEM's <br/>linearized <br/> backbones</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Assembly</h3></div></td> | ||
+ | |||
+ | <td class="white arrow" > | ||
+ | <p>3A assembly <br/>for D1-D3</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Stored <br/>in -20°C</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | </table> | ||
<h2 id="week2">Week 2</h2> | <h2 id="week2">Week 2</h2> | ||
+ | <table > | ||
+ | <tr> | ||
+ | <td class="bottomline rightline first"><h3 style="padding-top:0;margin-top:0;margin-bottom:0;padding-bottom:0;">Construct <br>worked with</h3></td> | ||
+ | <td class="bottomline"><h3>Things done</h3></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"></td> | ||
+ | <td class="bottomline"><h3>June 29th - <br/> July 7th</h3></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Preparations</h3></div></td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Transform <br/> AHD6 & AHD7 <br/>into TOP10</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>o/n culture</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>MinPrep</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3>Assembly</h3></div></td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Transform <br/>D1-D3 <br/>into TOP10</p> | ||
+ | </td> | ||
+ | |||
+ | <td class="blue arrow" > | ||
+ | <p>D2 <br/>expressing <br/>GFP</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Refresh <br/> positive <br/>colonies</p> | ||
+ | </td> | ||
+ | <td class="red arrow" > | ||
+ | <p>No GFP</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
+ | <td> | ||
+ | </td> | ||
+ | |||
+ | <td class="reddown arrow" > | ||
+ | <p>No colonies <br/> for D1 & D3</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Re-assemble D1-D3</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>Transform into TOP10</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>D2 <br/>successful</p> | ||
+ | </td> | ||
+ | <td class="white arrow" > | ||
+ | <p>o/n culture</p> | ||
+ | </td> | ||
+ | <td class="blue arrow" > | ||
+ | <p>MiniPrep</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="first rightline"><div class="heightdiv"><h3></h3></div></td> | ||
+ | <td> | ||
+ | </td> | ||
+ | |||
+ | <td> | ||
+ | </td> | ||
+ | <td> | ||
+ | </td> | ||
+ | <td> | ||
+ | </td> | ||
+ | <td class="reddown arrow" > | ||
+ | <p>No colonies <br/> for D1 & D3</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<h2 id="week3">Week 3</h2> | <h2 id="week3">Week 3</h2> | ||
<h2 id="week4">Week 4</h2> | <h2 id="week4">Week 4</h2> |
Revision as of 12:29, 12 August 2015