Difference between revisions of "Team:Paris Bettencourt/Project/VitaminA"

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<h3>June</h3>
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<h2>Vitamin A</h2>
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Brainstorming and reading literature about vitamin B12. What organism should we take the pathway from, and what organism should we put it in?<br><br>
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<b>Make <i>Lactobacillus plantarum</i> produce B12</b><br>
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The idea is to make an organism already present in idli (<i>Lactobacillus plantarum</i>) produce vitamin B12. A close cousin of this organism, <i>L. reuteri</i> was thought to produce B12 and had the advantage to have the cobalamin biosynthesis bathway in one single stretch of DNA.
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<b>Overexpress the B12 production pathway in <i>Lactobacillus reuteri</i></b><br>
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<h4>Bibliography</h4>
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The idea is to take an organism already present in idli (<i>Lactobacillus reuteri</i>) and to make it produce even more B12. Its pathway has been studied and we planned to metabolic engineer it as it had already been done in <i>Bacillus megaterium</i> (Biedendieck <i>et al.</i>, 2010).<br>
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The problem is that <i>L. reuteri</i> actually produces pseudo-cobalamin, which has not been proven to be active in mammals (<span style="color: red">Santos reference?</span>).<br>
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<li>Li, Q., Sun, Z., Li, J. & Zhang, Y. Enhancing beta-carotene production in Saccharomyces cerevisiae by metabolic engineering. <i>FEMS Microbiology Letters</i> <b>345</b>, 94-101 (2013).</li>
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So instead of overproducing a potentially non-active form of cobalamin, we thought of changing the pathway in <i>L. reuteri</i> to make it produce an active form of cobalamin. Unfortunately, due to an apparent difficulty and a lack of knowledge in the community, we dropped this idea.
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<li>Beekwilder, J. et al. Polycistronic expression of a ß-carotene biosynthetic pathway in Saccharomyces cerevisiae coupled to ß-ionone production. <i>Journal of Biotechnology</i> (2014).</li>
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<li>Voth, W.P., Richards, J.D., Shaw, J.M. & Stillman, D.J. Yeast vectors for integration at the HO locus. <i>Nucleic acids research</i> <b>29</b>, E59-E59 (2001).</li>
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<li>Gietz, R.D. & Schiestl, R.H. High-efficiency yeast transformation using the LiAc / SS carrier DNA / PEG method. <i>Nature Protocols</i> <b>2</b>, 31-35 (2008).</li>
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Revision as of 09:31, 13 August 2015

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Vitamin A




Bibliography

  • Li, Q., Sun, Z., Li, J. & Zhang, Y. Enhancing beta-carotene production in Saccharomyces cerevisiae by metabolic engineering. FEMS Microbiology Letters 345, 94-101 (2013).
  • Beekwilder, J. et al. Polycistronic expression of a ß-carotene biosynthetic pathway in Saccharomyces cerevisiae coupled to ß-ionone production. Journal of Biotechnology (2014).
  • Voth, W.P., Richards, J.D., Shaw, J.M. & Stillman, D.J. Yeast vectors for integration at the HO locus. Nucleic acids research 29, E59-E59 (2001).
  • Gietz, R.D. & Schiestl, R.H. High-efficiency yeast transformation using the LiAc / SS carrier DNA / PEG method. Nature Protocols 2, 31-35 (2008).

July

References

Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.

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