Difference between revisions of "Team:UiOslo Norway/Experiments/Alkaline Phospatase"
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Revision as of 08:12, 14 August 2015
Shrimp Alkaline Phosphatase Treatment (Fermentas):
Back to ProtocolsUse Zero blunt TOPO PCR cloning Kit
2 μl 10X Reaction Buffer 1 μl shrimp alkaline phosphatase x μl DNA( 1 μg total concentration) MilliQ up to 20 μl
Total Reaction of 20 μl
Incubate at 37 °C for 30 minutes Heat inactivation at 65 °C for 15 minutes.