Difference between revisions of "Team:UiOslo Norway/Experiments/Restriction Digest"

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5 μl  Cutsmart</br>
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x μl DNA (1 μg total concentration)</br>
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1 μl Restriction Enzyme I</br>
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1 μl Restriction Enzyme II</br>
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up to 50 μl  MilliQ Water</br>
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<p>Total Reaction of 50 μl </p>
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Incubate for 1 hour at 37 °C</br>
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For restriction digests including NdeI, at first only 1 μl of NdeI was added and the digest incubated at 37 °C for 1 hour, afterwards NdeI was heat inactivated at 65 °C for 20 minutes and then the second restriction enzyme was added and the digest was again incubated for 1 hour.
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Revision as of 08:18, 14 August 2015

Restriction Digest:

Back to Protocols


5 μl Cutsmart
x μl DNA (1 μg total concentration)
1 μl Restriction Enzyme I
1 μl Restriction Enzyme II
up to 50 μl MilliQ Water

Total Reaction of 50 μl


Incubate for 1 hour at 37 °C

For restriction digests including NdeI, at first only 1 μl of NdeI was added and the digest incubated at 37 °C for 1 hour, afterwards NdeI was heat inactivated at 65 °C for 20 minutes and then the second restriction enzyme was added and the digest was again incubated for 1 hour.