Difference between revisions of "Team:UiOslo Norway/Experiments/PCR taq"

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2.5 µl 10X Standard Taq Reaction buffer </br>
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2.5 µl dNTPS [2 mM]</br>
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0.5 µl Forward Primer [10 µM]</br>
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0.5 µl Reverse Primer  [10 µM]</br>
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1 µl Template DNA </br>
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0.125 µl Taq DNA Polymerase</br>
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17.875 µl MilliQ Water </br>
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<p>25 µl Total Reaction </p>
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Revision as of 08:24, 14 August 2015

PCR with Taq DNA Polymerase (NEB):

Back to Protocols


2.5 µl 10X Standard Taq Reaction buffer
2.5 µl dNTPS [2 mM]
0.5 µl Forward Primer [10 µM]
0.5 µl Reverse Primer [10 µM]
1 µl Template DNA
0.125 µl Taq DNA Polymerase
17.875 µl MilliQ Water

25 µl Total Reaction