Difference between revisions of "Team:UiOslo Norway/Experiments/NMS medium"
| Line 19: | Line 19: | ||
9.0 mM CaCl2</br> | 9.0 mM CaCl2</br> | ||
up to 1L Water </br></br> | up to 1L Water </br></br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
<b>Ethylenediaminetetraacetic acid ferric sodium salt</b></br> | <b>Ethylenediaminetetraacetic acid ferric sodium salt</b></br> | ||
103.5 mM FeEDTA</br> | 103.5 mM FeEDTA</br> | ||
up to 100 ml Water</br></br> | up to 100 ml Water</br></br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
<b>Sodium Molybdate dihydrate</b></br> | <b>Sodium Molybdate dihydrate</b></br> | ||
1.07 mM NaMoO4 *2H2O</br> | 1.07 mM NaMoO4 *2H2O</br> | ||
up to 500 ml Water</br></br> | up to 500 ml Water</br></br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
<b>Trace Elements solution</b></br> | <b>Trace Elements solution</b></br> | ||
0.8 mM CuSO4*5 H2O</br> | 0.8 mM CuSO4*5 H2O</br> | ||
| Line 46: | Line 40: | ||
</br> | </br> | ||
Store dark.</br> | Store dark.</br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
<b>Phosphate buffer</b></br> | <b>Phosphate buffer</b></br> | ||
198.25 mM Na2HPO4*12H2O</br> | 198.25 mM Na2HPO4*12H2O</br> | ||
| Line 55: | Line 47: | ||
</br> | </br> | ||
Add H2O to 800 ml, adjust pH to 6,8 and add H2O to 1000 ml. Autoclave or sterile filter.</br> | Add H2O to 800 ml, adjust pH to 6,8 and add H2O to 1000 ml. Autoclave or sterile filter.</br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
<b>Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate</b></br> | <b>Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate</b></br> | ||
400.5 mM CuSO4*5H2O</br> | 400.5 mM CuSO4*5H2O</br> | ||
up to 100 ml H2O</br></br> | up to 100 ml H2O</br></br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
<b>Iron(II) sulfate heptahydrate</b></br> | <b>Iron(II) sulfate heptahydrate</b></br> | ||
3.6 mM FeSO4*7H2O</br> | 3.6 mM FeSO4*7H2O</br> | ||
| Line 69: | Line 57: | ||
</br> | </br> | ||
Adjust to pH=3 after mixing to avoid precipitation.</br> | Adjust to pH=3 after mixing to avoid precipitation.</br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
<b>Zinc sulfate heptahydrate</b></br> | <b>Zinc sulfate heptahydrate</b></br> | ||
347.75 mM ZnSO4*7H2O</br> | 347.75 mM ZnSO4*7H2O</br> | ||
| Line 81: | Line 67: | ||
161.73 mM H3BO3</br> | 161.73 mM H3BO3</br> | ||
up to 100 ml Water</br></br> | up to 100 ml Water</br></br> | ||
| − | </ | + | </br> |
| − | + | ||
| − | + | ||
| − | + | ||
| + | <b>Cobalt(II) chloride hexahydrate</b></br> | ||
| + | 42.03mM CoCl2*6H2O</br> | ||
| + | up to 100 ml H2O</br> | ||
| + | </br> | ||
| + | <b>Manganese(II) chloride tetrahydrate</b></br> | ||
| + | 50.53 mM MnCl2*4H2O</br> | ||
| + | up to 100 ml</br> | ||
| + | </br> | ||
| + | <b>Nickel(II) chloride hexahydrate</b></br> | ||
| + | 42.07 mM</br> | ||
| + | up to 100 ml Water</br> | ||
| + | </br> | ||
| + | </p> | ||
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Revision as of 11:01, 14 August 2015
NMS medium
Back to Protocols10x NMS 98.9 mM KNO3 43.8 mM MgSO4 * 6 H2O 9.0 mM CaCl2 up to 1L Water Ethylenediaminetetraacetic acid ferric sodium salt 103.5 mM FeEDTA up to 100 ml Water Sodium Molybdate dihydrate 1.07 mM NaMoO4 *2H2O up to 500 ml Water Trace Elements solution 0.8 mM CuSO4*5 H2O 1.8 mM FeSO4*7H2O 1.4 mM ZnSO4*7H2O 0.24 mM H3BO3 0.21 mM CoCl2*6H2O 0.74 mM EDTA-Na2 0.1 mM MnCL2*4H2O 42.07 µM NiCl2*6H2O up to 500 ml Water Store dark. Phosphate buffer 198.25 mM Na2HPO4*12H2O 191.05 mM KH2PO4 up to 100 ml Water Add H2O to 800 ml, adjust pH to 6,8 and add H2O to 1000 ml. Autoclave or sterile filter. Preparation of solutions for Trace elements solution Copper(II) sulfate pentahydrate 400.5 mM CuSO4*5H2O up to 100 ml H2O Iron(II) sulfate heptahydrate 3.6 mM FeSO4*7H2O up to 1L Water Adjust to pH=3 after mixing to avoid precipitation. Zinc sulfate heptahydrate 347.75 mM ZnSO4*7H2O up to 100 ml Water
Boric Acid 161.73 mM H3BO3 up to 100 ml Water Cobalt(II) chloride hexahydrate 42.03mM CoCl2*6H2O up to 100 ml H2O Manganese(II) chloride tetrahydrate 50.53 mM MnCl2*4H2O up to 100 ml Nickel(II) chloride hexahydrate 42.07 mM up to 100 ml Water