Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Notebook"

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<div class="Text1">July</div>
 
<div class="Text1">July</div>
  
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<div class="Container2"><div class="Text2" id="First1">7/4</div></div>
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<div class="Container2"><div class="Text2">7/4</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Green>Manufacture <i>CP<Super>+</Super></i> plate</Green>
 
<Green>Manufacture <i>CP<Super>+</Super></i> plate</Green>
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<div class="Container2"><div class="Text2" id="First1">7/6</div></div>
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<div class="Container2"><div class="Text2">7/6</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Red>Learn how to transform</Red>
 
<Red>Learn how to transform</Red>
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<div class="Container2"><div class="Text2" id="First1">7/7</div></div>
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<div class="Container2"><div class="Text2">7/7</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Red>Learn how to manufacture competent cell</Red>
 
<Red>Learn how to manufacture competent cell</Red>
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<div class="Container2"><div class="Text2" id="First1">7/8</div></div>
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<div class="Container2"><div class="Text2">7/8</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Red>Learn how to extract the plasmid</Red>
 
<Red>Learn how to extract the plasmid</Red>
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<div class="Container2"><div class="Text2" id="First1">7/9</div></div>
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<div class="Container2"><div class="Text2">7/9</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Green>Apoptosis part:</Green>
 
<Green>Apoptosis part:</Green>
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<div class="Container2"><div class="Text2" id="First1">7/10</div></div>
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<div class="Container2"><div class="Text2">7/10</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Blue>Manufacture <i>CP<Super>+</Super></i> plate</Blue>
 
<Blue>Manufacture <i>CP<Super>+</Super></i> plate</Blue>
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<div class="Container2"><div class="Text2" id="First1">7/11</div></div>
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<div class="Container2"><div class="Text2">7/11</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Green>Apoptosis part:</Green>
 
<Green>Apoptosis part:</Green>
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<div class="Container2"><div class="Text2" id="First1">7/12</div></div>
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<div class="Container2"><div class="Text2">7/12</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Green>Apoptosis part:</Green>
 
<Green>Apoptosis part:</Green>
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<div class="Container2"><div class="Text2" id="First1">7/13</div></div>
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<div class="Container2"><div class="Text2">7/13</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Green>PYY part:</Green>
 
<Green>PYY part:</Green>
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<div class="Container2"><div class="Text2" id="First1">7/20</div></div>
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<div class="Container2"><div class="Text2">7/20</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Red>Discuss about total synthesis</Red>
 
<Red>Discuss about total synthesis</Red>
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<div class="Container2"><div class="Text2" id="First1">7/13</div></div>
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<div class="Text3">
 
<div class="Text3">
 
<Green>PYY part:</Green>
 
<Green>PYY part:</Green>
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<div class="Container2"><div class="Text2" id="First1">7/24</div></div>
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<div class="Container2"><div class="Text2">7/24</div></div>
 
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<div class="Text3">
 
<Red>Discuss our project with professor</Red>
 
<Red>Discuss our project with professor</Red>
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<div class="Container2"><div class="Text2" id="First1">7/25</div></div>
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<div class="Container2"><div class="Text2">7/25</div></div>
 
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<div class="Text3">
 
<Red>Discuss our problems with Instructor</Red>
 
<Red>Discuss our problems with Instructor</Red>
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<div class="Container2"><div class="Text2" id="First1">7/26</div></div>
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<div class="Container2"><div class="Text2">7/26</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Green>PYY part:</Green>
 
<Green>PYY part:</Green>
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<div class="Container2"><div class="Text2" id="First1">7/27</div></div>
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<div class="Container2"><div class="Text2">7/27</div></div>
 
<div class="Text3">
 
<div class="Text3">
 
<Green>PYY part:</Green>
 
<Green>PYY part:</Green>

Revision as of 06:47, 17 August 2015

NTU-LIHPAO-Taiwan

July
7/4
Manufacture CP+ plate
7/6
Learn how to transform
Apoptosis part:
Transformation of:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
7/7
Learn how to manufacture competent cell
Manufacture competent cell
PYY part:
Transformation of:
[promoter] (BBa_J23100), [signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Apoptosis part:
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806), [C1] (BBa_C0051)
7/8
Learn how to extract the plasmid
Learn how to conduct the gel extraction and electrophoresis
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C2] (BBa_C0053)
Restriction enzyme:
[RBS] cleave by EcoRI, [C2] cleave by EcoRI and PstI
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C2] ----- Result: Succeeded
Transformation of:
[pC1] (BBa_R0051), [pC2] (BBa_R0053), [endolysin] (BBa_K112806) again
7/9
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
Gel electrophoresis:
[RBS] ----- Result: Failed (Smeared band was too blurred)
[C1] ----- Result: Succeeded
[endolysin] ----- Result: Failed (Smeared band was too blurred)
PYY part:
Extract the plasmid:
[signal peptide] (BBa_K554002), [terminator] (BBa_B0015)
Gel electrophoresis:
[signal peptide] ----- Result: Succeeded
[terminator] ----- Result: Succeeded
7/10
Manufacture CP+ plate
Manufacture Amp+ plate
Apoptosis part:
Gel electrophoresis:
[RBS] (BBa_B0034) ----- Result: Failed (Smeared band was too blurred)
[C2] (BBa_C0053) ----- Result: Succeeded
Gel extraction:
[C2]
7/11
Apoptosis part:
Extract the plasmid:
[RBS] (BBa_B0034), [C1] (BBa_C0051), [endolysin] (BBa_K112806)
PYY part:
Transformation of:
[promoter] (BBa_J23101)
7/12
Apoptosis part:
Extract the plasmid:
[pC1] (BBa_R0051)
7/13
PYY part:
Extract the plasmid:
[promoter] (BBa_J23101)
Gel electrophoresis (two white colonies & two red colonies):
[promoter] ------ Result: Failed (white colonies didn’t have band, red colonies had band but not the correct size, we speculated that was pollution)
7/20
Discuss about total synthesis
7/13
PYY part:
Transformation of:
[terminator] (BBa_B0015)
7/24
Discuss our project with professor
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Failed
7/25
Discuss our problems with Instructor
7/26
PYY part:
Cultured [terminator] again
7/27
PYY part:
Extract the plasmid:
[terminator] (BBa_B0015)
Gel electrophoresis:
[terminator] ----- Result: Succeeded