Difference between revisions of "Team:Warwick/Project5"
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<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
<h2>Week 5</h2> | <h2>Week 5</h2> | ||
| − | <p> | + | <p>Both of the chemically transformed plates and electrotransformed plate grew over weekend. |
| + | <br>LppompAzif268: colony PCR of clone 2 ok (inoculate culture to purify DNA inoculate 2 | ||
| + | |||
| + | cultures, 4.5ml each. Miniprep them after making a glycerol stock). | ||
| + | <br>Conducted plasmid miniprep of bacterial culture. Gel electrophoresis of redone PCR Pgsa worked, zf2 (all others need to be repeated at | ||
| + | |||
| + | a later stage). Running gel of miniprep restriction digestion. Redoing PCR: INP, zf2, zf14 (67oC). zf10 (69oC) with DMSO (0%, 3%, 6%). PCR purification of BCLA and PGSA (nanodrop returned negative, redoing). Redoing PCR of INP, zf2, zf10, zf14. | ||
| + | <br>Redid PCR of INP, zf10 and zf14. zf14 returned positive, purified (31.8 ng/l. Redoing PCR of INP and zf10. Purified zf2, nanodropped. | ||
| + | </p> | ||
<a href="#0" class="cd-read-more">Read more</a> | <a href="#0" class="cd-read-more">Read more</a> | ||
<span class="cd-date">Jul 27</span> | <span class="cd-date">Jul 27</span> | ||
Revision as of 12:21, 19 August 2015
