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| | <h1 id="Notebook">Notebook</h1> | | <h1 id="Notebook">Notebook</h1> |
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| − | ###``` GET /protocols/:id ```
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| − | <b>Response</b>
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| − |
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| − | ```
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| − | {
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| − | "id":"beta-glactasidase-stain",
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| − | "url":"https://www.scientificprotocols.org/api/v1/protocols/beta-glactasidase-stain",
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| − | "title":"Beta-Glactasidase Stain",
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| − | "description":"\r\n\r\n### Solutions\r\n\r\n1. Solution A\r\n - 5mM potassium ferricyanide, crystalline\r\n - 5mM potassium ferrocyanide, trihydrate\r\n- X-gal Stock Solution\r\n - 40mg/mL in DMSO (100mg in 2.5ml DMSO)\r\n- Final X-gal Solution\r\n - Dilute X-gal Stock Solution 1:30 in Solution A\r\n - (warm Solution A to 37°C to prevent precipitation of X-gal)\r\n\r\n\r\n### Staining Protocol\r\n\r\nStart with fixed frozen 10μm tissue sections on pap-penned slides\r\n\r\n1. Allow slides to completely dry for 1 hour\r\n- Fix tissue with 2% formaldehyde + 0.2% glutaraldehyde for 5 min.\r\n- Rinse with 1xPBS\r\n- Incubate slides in x-gal solution in humidity chamber at 37°C overnight\r\n- Rinse with 1xPBS\r\n- Immerse slides in 100% ethanol until all precipitate dissolves (1-3 hours)\r\n- Place slides in:\r\n - a. Eosin Y 25 seconds\r\n - b. 95% Ethanol 1 minute\r\n - c. 100% Ethanol 1 minute\r\n - d. 100% Ethanol 1 minute\r\n - e. 100% Ethanol 1 minute\r\n - f. Xylene 2 minutes\r\n - g. Xylene 10 dips\r\n- Coverslip slides using Cytoseal 60\r\n\r\n",
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| − | "gist_id":"315e630a1fe9fe6e9bab",
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| − | "html_url":"https://www.scientificprotocols.org/protocols/beta-glactasidase-stain",
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| − | "discussion_html_url":"https://www.scientificprotocols.org/protocols/beta-glactasidase-stain/discussion",
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| − | "tags":[
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| − | "Histology"
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| − | ],
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| − | "average_rating":4,
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| − | "number_of_ratings":3,
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| − | "doi":"10.5281/zenodo.13491",
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| − | "citation_html_url":"http://dx.doi.org/10.5281/zenodo.13491",
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| − | "author":{
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| − | "username":"scientificprotocols",
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| − | "html_url":"https://www.scientificprotocols.org/users/scientificprotocols"
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| − | },
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| − | "created_at":"2014-07-09T18:59:17.254Z",
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| − | "updated_at":"2014-07-09T18:59:17.254Z"
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| − | }
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| − | ```
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| | | | |
| | </div> | | </div> |