Difference between revisions of "Team:UFSCar-Brasil"

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<div class="pusher">
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    <!-- Menu -->
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      <div class="ui center aligned container">
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        <div class="item">
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        <a href="/index.html" class="item">Home</a>
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            <a class="item">Overview</a>
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            <div class="divider"></div>
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            <a class="item">Problem</a>
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            <a class="item">Plasmolise</a>
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            <a class="item">Limoneno sintase</a>
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            <a class="item">Manufactoring</a>
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          </div>
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        <a class="item">Team</a>
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        <div class="ui dropdown item">
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          Achievments
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            <a href="#" class="item">Overview</a>
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            <a href="#" class="item">Parts</a>
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            <a href="#" class="item">Results</a>
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            <a href="#" class="item">Judging Criteria</a>
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        <div class="ui dropdown item">
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          Policy & Practices
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            <a href="#" class="item">Overview</a>
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            <div class="divider"></div>
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            <a href="#" class="item">Political</a>
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            <a href="#" class="item">Educacional</a>
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            <a href="#" class="item">Environment</a>
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            <a href="#" class="item">BioBricks</a>
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            <a href="#" class="item">Meeting</a>
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          Modelling
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            <a href="#" class="item">Overview</a>
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            <a href="#" class="item">Part I</a>
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            <a href="#" class="item">Part II</a>
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            <a href="#" class="item">Part III</a>
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            <a href="#" class="item">Protocols</a>
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    <!-- /Menu -->
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            <img class ="ui fluid image" src="img/banner-4.jpg" alt="">
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          <h1 class="ui inverted header">
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            UFSCar - Brasil
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          </h1>
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          <h2>People helping people</h2>
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          <div class="ui huge inverted primary basic button">See our project <i class="right arrow icon"></i></div>
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            <h3 class="ui header">The Project</h3>
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            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquam voluptate nihil nesciunt ut minus atque eaque voluptates provident laboriosam explicabo quia eligendi quam vero esse quos vel, mollitia nobis eveniet.</p>
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            <h3 class="ui header">Something</h3>
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            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Deleniti temporibus ullam minus hic labore quaerat, nisi optio, earum illo sapiente assumenda molestias minima voluptatibus. Saepe laudantium soluta error veniam alias.</p>
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            <a class="ui huge button">Check our project!</a>
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            <h3 class="ui header">The Team</h3>
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            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Aliquam voluptate nihil nesciunt ut minus atque eaque voluptates provident laboriosam explicabo quia eligendi quam vero esse quos vel, mollitia nobis eveniet.</p>
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            <h3 class="ui header">Something</h3>
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            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Deleniti temporibus ullam minus hic labore quaerat, nisi optio, earum illo sapiente assumenda molestias minima voluptatibus. Saepe laudantium soluta error veniam alias.</p>
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            <a class="ui huge button">Check our team!</a>
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    <div class="ui vertical stripe segment">
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      <div class="ui text container">
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        <h3 class="ui header">Breaking The Grid, Grabs Your Attention</h3>
 +
        <p>Instead of focusing on content creation and hard work, we have learned how to master the art of doing nothing by providing massive amounts of whitespace and generic content that can seem massive, monolithic and worth your attention.</p>
 +
        <a class="ui large button">Read More</a>
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        <h4 class="ui horizontal header divider">
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          <a href="#">Case Studies</a>
 +
        </h4>
 +
        <h3 class="ui header">Lorem ipsum?</h3>
 +
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto aperiam ratione officiis ex error deleniti, numquam odio blanditiis vero similique nam reprehenderit quis enim culpa deserunt iure. Quas, harum, esse.</p>
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        <a class="ui large button">I'm Still Quite Interested</a>
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        <h3 class="ui center aligned header">Our amazing sponsors!</h3>
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            <h4 class="ui inverted header">About</h4>
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              <a href="#" class="item">Sitemap</a>
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              <a href="#" class="item">Contact Us</a>
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              <a href="#" class="item">Religious Ceremonies</a>
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              <a href="#" class="item">Gazebo Plans</a>
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              <a href="#" class="item">Pre-Order</a>
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              <a href="#" class="item">DNA FAQ</a>
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              <a href="#" class="item">How To Access</a>
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              <a href="#" class="item">Favorite</a>
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          <div class="seven wide column">
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            <h4 class="ui inverted header">Footer Header</h4>
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            <p>Extra space for a call to action inside the footer that could help re-engage users.</p>
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          </div>
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        </div>
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      </div>
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  </div>
 
<h2> Project Description </h2>
 
<h2> Project Description </h2>
 
<p align="justify">Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem, our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET (N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared to other products and the replacement of the compound DEET  by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>
 
<p align="justify">Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem, our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET (N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared to other products and the replacement of the compound DEET  by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.</p>

Revision as of 05:06, 25 August 2015

Home
Project
Overview
Problem Plasmolise Limoneno sintase Manufactoring
Team
Achievments
Overview
Parts Results Judging Criteria
Policy & Practices
Overview
Political Social Educacional Environment BioBricks Meeting Fundraising
Modelling
Overview
Part I Part II Part III
Notebook
Overview
Protocols Survey
Safety
About our labs About our project

UFSCar - Brasil

People helping people

See our project

The Project

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Something

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Check our project!

The Team

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Something

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Check our team!

Breaking The Grid, Grabs Your Attention

Instead of focusing on content creation and hard work, we have learned how to master the art of doing nothing by providing massive amounts of whitespace and generic content that can seem massive, monolithic and worth your attention.

Read More

Case Studies

Lorem ipsum?

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto aperiam ratione officiis ex error deleniti, numquam odio blanditiis vero similique nam reprehenderit quis enim culpa deserunt iure. Quas, harum, esse.

I'm Still Quite Interested

Our amazing sponsors!

About

Sitemap Contact Us Religious Ceremonies Gazebo Plans

Services

Pre-Order DNA FAQ How To Access Favorite

Footer Header

Extra space for a call to action inside the footer that could help re-engage users.

Project Description

Diseases transmitted by insect vectors, such as malaria and dengue, affect significantly the Brazilian population. In our city, São Carlos, this year in summer the public health organ featured a big number of cases. Working in this problem, our project consists in the development of an alternative repellent of that currently sold in the market and more or equally effective in preventing mosquitoes bites transmitted diseases. The main compound in current repellents is DEET (N, N-diethyl-m-toluamide), a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on their use in products. The main characteristic of our repellent is the long duration when compared to other products and the replacement of the compound DEET by D-limonene. Instead, the D-limonene has low toxicity, is highly volatile and present a pleasant smell.

Our proposal is building a bacteria carrying out the production of D-limonene via limonene synthase. To enable long term storage at room temperatures, the bacterial cells that make up the repellent will be plasmolyzed, with suspended metabolism, considered a dormant state. The maintenance of this state will be obtained by a solution of polyethyleneglycol (PEG) that will raise the osmotic pressures. Once in contact with the skin, the PEG solution will be diluted by sweat, inducing an osmotic shock in cells. Then the universal stress protein promoter (UspA) will be activated and will induce the expression of limonene synthase. Beyond PEG, other compounds such as glycerol and metal ions in low concentrations will compose our insect repellent cream, in order to sustain the bacteria to the required metabolites building and posterior enzyme activities. In this way, the distribution of our repellent may become feasible, allowing its use.

To overcome those problems with limonene synthase folding, we used constitutive promoters for the expression of chaperones from all Escherichia coli available classes (like ClpB, DnaK and IbpA / IbpB). It will reinforce the stockpile of chaperones naturally produced during osmotic shocks (heat-shock proteins) in bacteria. Our goal is to improve protein solubility, indirectly creatinga toolkit for protein solubility enhancement for future iGEM teams. . Besides, reducing the occurrence of insoluble bodies and improving the production of limonene.

Finally, a way to ensure the biosafety in the use of our repellent and keep a control of bacterial cells is the use of a system of programmed death or Kill Switch. This system will work with two suicide genes: Killer red, a protein which trigger oxidative species generation destroying the DNA (plasmid and chromosomal) allied to a potent RNAse which will avoid any contribution of RNA to host or normal microbiota. These genes will be switch on through an association with a zinc sensitive promoter triggered by zur proteins which associate with zinc and avoid the expression of them allowing the bacterial growing. After long periods of repellent action, bacteria activities will decrease zinc concentration in medium, which allows the gene activation and cells death.