Difference between revisions of "Team:TU Dresden/Project/Description"

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<h1>About us</h1>
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Our team consists of 11 students and 2 supervisors (Jana Herrmann and Frank Gro&szlig;). We are biologists, bioengineers and biochemists. If you want to know a little bit more about us, you can read some information on this site.
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<h1>Description</h1>
 
  
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<h2>Introduction</h2>
 
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<p style="line-height:1.8">Our project is called <b>SPACE-P</b> (Structural Phage Assisted Continuous Evolution of Proteins) and is part of the Synthetic Biology field. It focuses on accelerating the process of developing protein binding partners, which is required in pharmaceutical research as well as biotechnology and many other fields of science. Thus far phage display is the most commonly used method for the discovery of protein binding partners. In this method a large library of potential binding partners is created where the strongest candidates are selected for further affinity testing. This process is very time consuming, cost intensive, requires human intervention steps, and is limited to the size of the given library. Our platform will make it possible to start with a single molecule, transform it by mutation and selection pressure to improve the binding affinity towards a target protein. This method is not only easy to implement, utilizing simple organisms and devices, but also significantly faster and cheaper than currently used tools. </p>
 
  
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<h2>Background</h2>
 
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<h3>PACE</h3>
 
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<p style="line-height:1.8">Phage assisted continuous evolution or PACE is a system designed for the continuous, directed evolution of biomolecules. The principle is that E. coli flow through a lagoon in which M13 are present and viable.  Important to note is that the flow rate of the <i>Escherichia coli</i> (<i>E. coli</i> is adjusted so that it is faster than their doubling time but not of that of the M13. Another important aspect of this setup is the deletion of gene P3 on M13, which is necessary for infection and proliferation. Instead the gene P3 of the M13 is encoded on the <i>E. coli</i> plasmid under the control of an upstream activating sequence (UAS). To undergo further infection cycles, the initial infectious generation of transgenic phage must activate the UAS by binding their activating domain (AD) to the binding domain (BD). This requires favorable protein-protein interactions between an X provided by the M13 and Y provided by the UAS of the E.Coli. As a result, and some additional help from a mutagenesis plasmid, M13 evolves this interaction between X and Yin order to stay in the lagoon. A stronger interaction creates a selection advantage and will be favored over a weaker interaction.</p>
 
  
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<p style="line-height:1.8">Jana studied Molecular Biotechnology in Dresden and Munich. From biophysics to biochemistry to chemistry, she has been working on projects in different disciplines. However, her favorite discipline has always been Molecular Bioengineering. Therefore, she is happy to support the TU Dresden team to get SPACE-P into the orbit of iGEM. Being outside the lab, she enjoys the nature while hiking or cycling, and for working off energy – if something is left at the end of a busy week – she likes playing badminton. She says: “The iGEM competition is a great opportunity for the students to get a feeling of what it takes to plan and perform a project (also from the organizational and financial side) and to realize that having know-how is beneficial, but having a good network is as important.”</p>

Revision as of 12:16, 26 August 2015


About us

Our team consists of 11 students and 2 supervisors (Jana Herrmann and Frank Groß). We are biologists, bioengineers and biochemists. If you want to know a little bit more about us, you can read some information on this site.

Jana

Jana studied Molecular Biotechnology in Dresden and Munich. From biophysics to biochemistry to chemistry, she has been working on projects in different disciplines. However, her favorite discipline has always been Molecular Bioengineering. Therefore, she is happy to support the TU Dresden team to get SPACE-P into the orbit of iGEM. Being outside the lab, she enjoys the nature while hiking or cycling, and for working off energy – if something is left at the end of a busy week – she likes playing badminton. She says: “The iGEM competition is a great opportunity for the students to get a feeling of what it takes to plan and perform a project (also from the organizational and financial side) and to realize that having know-how is beneficial, but having a good network is as important.”