Difference between revisions of "Team:UCLA/Notebook"
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| Line 61: | Line 61: | ||
</details> | </details> | ||
<details> | <details> | ||
| − | <summary>mm/dd: Sample Entry | + | <summary>mm/dd: Sample Entry</summary> |
<p>Today we began cloning our GFP.</p> | <p>Today we began cloning our GFP.</p> | ||
<ul> | <ul> | ||
| Line 69: | Line 69: | ||
<li>Ligated into backbone</li> | <li>Ligated into backbone</li> | ||
</ul> | </ul> | ||
| + | <p>For our ligation, we made the following modifications:</p> | ||
| + | <ol> | ||
| + | <li>Tried it with newly bought ligase</li> | ||
| + | <li>Left reaction overnight instead of 2 hrs</li> | ||
| + | <li>Vector to insert ratio was 1:5 instead of 1:3</li> | ||
| + | </ol> | ||
<p>PCR Reaction:</p> | <p>PCR Reaction:</p> | ||
<table style="width: 50%; text-align: center"> | <table style="width: 50%; text-align: center"> | ||
| Line 167: | Line 173: | ||
</details> | </details> | ||
<details> | <details> | ||
| − | <summary>mm/dd: Sample Entry | + | <summary>mm/dd: Sample Entry</summary> |
<p>Today we began cloning our GFP.</p> | <p>Today we began cloning our GFP.</p> | ||
<ul> | <ul> | ||
| Line 175: | Line 181: | ||
<li>Ligated into backbone</li> | <li>Ligated into backbone</li> | ||
</ul> | </ul> | ||
| + | <p>For our ligation, we made the following modifications:</p> | ||
| + | <ol> | ||
| + | <li>Tried it with newly bought ligase</li> | ||
| + | <li>Left reaction overnight instead of 2 hrs</li> | ||
| + | <li>Vector to insert ratio was 1:5 instead of 1:3</li> | ||
| + | </ol> | ||
<p>PCR Reaction:</p> | <p>PCR Reaction:</p> | ||
<table style="width: 50%; text-align: center"> | <table style="width: 50%; text-align: center"> | ||
| Line 272: | Line 284: | ||
</details> | </details> | ||
<details> | <details> | ||
| − | <summary>mm/dd: Sample Entry | + | <summary>mm/dd: Sample Entry</summary> |
<p>Today we began cloning our GFP.</p> | <p>Today we began cloning our GFP.</p> | ||
<ul> | <ul> | ||
| Line 326: | Line 338: | ||
</details> | </details> | ||
</details> | </details> | ||
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| + | |||
Revision as of 09:02, 14 May 2015
Notebook Template
mm/dd - mm/dd (most recent weeks on top)
mm/dd: Entry title/short description (most recent days on top)
Plain text for descriptions, etc.
Unordered lists for bullet points
- Bulletpoint 1
- Bulletpoint 2
- Bulletpoint 3
For images, upload image onto wiki server. Then replace placeholder image url with your image's url.
Ordered lists for numbers
- Number 1
- Number 2
- Number 3
For tables, "tr" stands for "table row", and "td" means "table column."
| Row 1, Column 1 | Row 1, Column 2 | Row 1, Column 3 | Row 1, Column 4 |
| Row 2, Column 1 | Row 2, Column 2 | Row 2, Column 3 | Row 2, Column 4 |
| Row 1, Column 1 | Row 2, Column 2 | Row 3, Column 3 | Row 4, Column 4 |
For different types of text styling... bold, italics, underlined. For headers...
Headers
Smaller Header
You get the point
Feel free to google any other styling.
Keep all your html within the detail tags for the day/week you're putting your entry for.
To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html
mm/dd: Sample Entry
Today we began cloning our GFP.
- PCR'd off template
- Ran gel
- Restriction digest
- Ligated into backbone
For our ligation, we made the following modifications:
- Tried it with newly bought ligase
- Left reaction overnight instead of 2 hrs
- Vector to insert ratio was 1:5 instead of 1:3
PCR Reaction:
| Component | Volume |
| 5X Q5 Reaction Buffer | 5 |
| 10 mM dNTPs | 0.5 |
| 10 uM Forward (primer 3/7) | 1.25 |
| 10 uM Reverse (primer 8) | 1.25 |
| Template (diluted to 1ng/uL) | 0.5 |
| Q5 High Fidelity DNA Polymerase | 0.25 |
| Nuclease Free Water | 16.25 |
Gel: Lot of bands, all at correct sizes
mm/dd - mm/dd (most recent weeks on top)
mm/dd: Entry title/short description (most recent days on top)
Plain text for descriptions, etc.
Unordered lists for bullet points
- Bulletpoint 1
- Bulletpoint 2
- Bulletpoint 3
For images, upload image onto wiki server. Then replace placeholder image url with your image's url.
Ordered lists for numbers
- Number 1
- Number 2
- Number 3
For tables, "tr" stands for "table row", and "td" means "table column."
| Row 1, Column 1 | Row 1, Column 2 | Row 1, Column 3 | Row 1, Column 4 |
| Row 2, Column 1 | Row 2, Column 2 | Row 2, Column 3 | Row 2, Column 4 |
| Row 1, Column 1 | Row 2, Column 2 | Row 3, Column 3 | Row 4, Column 4 |
For different types of text styling... bold, italics, underlined. For headers...
Headers
Smaller Header
You get the point
Feel free to google any other styling.
Keep all your html within the detail tags for the day/week you're putting your entry for.
To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html
mm/dd: Sample Entry
Today we began cloning our GFP.
- PCR'd off template
- Ran gel
- Restriction digest
- Ligated into backbone
For our ligation, we made the following modifications:
- Tried it with newly bought ligase
- Left reaction overnight instead of 2 hrs
- Vector to insert ratio was 1:5 instead of 1:3
PCR Reaction:
| Component | Volume |
| 5X Q5 Reaction Buffer | 5 |
| 10 mM dNTPs | 0.5 |
| 10 uM Forward (primer 3/7) | 1.25 |
| 10 uM Reverse (primer 8) | 1.25 |
| Template (diluted to 1ng/uL) | 0.5 |
| Q5 High Fidelity DNA Polymerase | 0.25 |
| Nuclease Free Water | 16.25 |
Gel: Lot of bands, all at correct sizes
mm/dd - mm/dd (most recent weeks on top)
mm/dd: Entry title/short description (most recent days on top)
Plain text for descriptions, etc.
Unordered lists for bullet points
- Bulletpoint 1
- Bulletpoint 2
- Bulletpoint 3
For images, upload image onto wiki server. Then replace placeholder image url with your image's url.
Ordered lists for numbers
- Number 1
- Number 2
- Number 3
For tables, "tr" stands for "table row", and "td" means "table column."
| Row 1, Column 1 | Row 1, Column 2 | Row 1, Column 3 | Row 1, Column 4 |
| Row 2, Column 1 | Row 2, Column 2 | Row 2, Column 3 | Row 2, Column 4 |
| Row 1, Column 1 | Row 2, Column 2 | Row 3, Column 3 | Row 4, Column 4 |
For different types of text styling... bold, italics, underlined. For headers...
Headers
Smaller Header
You get the point
Feel free to google any other styling.
Keep all your html within the detail tags for the day/week you're putting your entry for.
To properly indent your html code, use: http://www.freeformatter.com/html-formatter.html
mm/dd: Sample Entry
Today we began cloning our GFP.
- PCR'd off template
- Ran gel
- Restriction digest
- Ligated into backbone
For our ligation, we made the following modifications:
- Tried it with newly bought ligase
- Left reaction overnight instead of 2 hrs
- Vector to insert ratio was 1:5 instead of 1:3
PCR Reaction:
| Component | Volume |
| 5X Q5 Reaction Buffer | 5 |
| 10 mM dNTPs | 0.5 |
| 10 uM Forward (primer 3/7) | 1.25 |
| 10 uM Reverse (primer 8) | 1.25 |
| Template (diluted to 1ng/uL) | 0.5 |
| Q5 High Fidelity DNA Polymerase | 0.25 |
| Nuclease Free Water | 16.25 |
Gel: Lot of bands, all at correct sizes