|
|
| Line 13: |
Line 13: |
| | <div id="redbox"> | | <div id="redbox"> |
| | <div style = "text-align: center;"><img width="300px" src="https://static.igem.org/mediawiki/2014/1/1d/Timeline-header.png"></div><br> | | <div style = "text-align: center;"><img width="300px" src="https://static.igem.org/mediawiki/2014/1/1d/Timeline-header.png"></div><br> |
| − | <iframe src=”https://www.dayhaps.com/en/widget-calendar-2360.html?lang=en” height=’50%’ width=’50%’ border=’0′></iframe>
| |
| − | <div id="textbox">
| |
| − | <h3 style= "text-align: center;">Week 1</h3>
| |
| − | <ol>
| |
| − | <li>Molecolar Biology Training Workshop</li>
| |
| − | <li>Practiced the basics of molecular cloning</li>
| |
| − | </ol>
| |
| − | <h3 style= "text-align: center;">Week 2</h3>
| |
| − | <ol>
| |
| − | <li>Idea Brainstorming and Generation</li>
| |
| − | <li>Compiled a preliminary list of potential ideas</li>
| |
| − | </ol>
| |
| − | <h3 style= "text-align: center;">Week 3</h3>
| |
| − | <ol>
| |
| − | <li>Settled on two main ideas: 1. Quorum sensing with antibiotics 2. Heavy metal removal with Magnetotactic bacteria</li>
| |
| − | <li>Learned how to use Geneious to design cloning process</li>
| |
| − | <li>Practiced extracting biobricks and transforming NEB Turbo cells with plasmids</li>
| |
| | | | |
| − | <li>Human Practices</li>
| + | <div id="calendar"> |
| − | <li>Visited Biomeme to get the portable qPCR machine</li>
| + | <div class="clasp hole-left"></div> |
| − | </ol>
| + | <div class="clasp hole-right"></div> |
| − | <h3 style= "text-align: center;">Week 4</h3>
| + | <div class="hole hole-left"></div> |
| − | <ol> | + | <div class="hole hole-right"></div> |
| − | <li>Decided on the project idea: Heavy metal removal with Magnetotactic bacteria
| + | <div id="month">« March 2012 »</div> |
| − | <li>Identified primers and promoters in AMB-1 strain
| + | <div id="days"> |
| − | <li>Identified AMB-1 transformation vector
| + | <table class="days"> |
| − | <li>Extracted smtA biobrick gene and made glycerol stock
| + | <tr> |
| − | </ol>
| + | <td>SUN</td> |
| − | <h3 style= "text-align: center;">Week 5</h3>
| + | <td>MON</td> |
| − | <ol> | + | <td>TUE</td> |
| − | <li>Developed three goals to accomplish for the project
| + | <td>WED</td> |
| − | <li>Determined the constructs to clone into AMB-1
| + | <td>THU</td> |
| − | <li>Designed two fast-fail experiments
| + | <td>FRI</td> |
| − | <li> Created a workflow for the construction of plasmid
| + | <td>SAT</td> |
| − | | + | </tr> |
| − | <li> Human Practices
| + | </table> |
| − | <li>Planned outreach events at high school summer programs
| + | </div> |
| − | </ol>
| + | <table class="day"> |
| − | <h3 style= "text-align: center;">Week 6</h3>
| + | <tr> |
| − | <ol> | + | <td class="old-month">26</td> |
| − | <li>Ordered chemicals for AMB-1 growth medium
| + | <td class="old-month">27</td> |
| − | <li>Completed primer design
| + | <td class="old-month">28</td> |
| − | <li>Obtained AMB-1 strain from Dr.Goolian
| + | <td class="old-month">29</td> |
| − | </ol>
| + | <td>1</td> |
| − | <h3 style= "text-align: center;">Week 7</h3>
| + | <td>2</td> |
| − | <ol> | + | <td class="selected">3</td> |
| − | <li>Determined the OD600 plate reading conversion formola experimentally
| + | </tr> |
| − | <li>Attempted to do E.Coli and AMB-1 cadmium tolerance test
| + | <tr> |
| − | <li>Designed all construct on Geneious
| + | <td>4</td> |
| − | | + | <td class="appoint">5</td> |
| − | <li>Human Practices
| + | <td>6</td> |
| − | <li>Met with Dr.Rizk to discuss presenting to incoming bioengineering freshmen
| + | <td>7</td> |
| − | </ol>
| + | <td>8</td> |
| − | <h3 style= "text-align: center;">Week 8</h3>
| + | <td>9</td> |
| − | <ol> | + | <td>10</td> |
| − | <li>Ordered DNAs from Genscript and IDT
| + | </tr> |
| − | </ol>
| + | <tr> |
| − | <h3 style= "text-align: center;">Week 9</h3>
| + | <td>11</td> |
| − | <ol> | + | <td>12</td> |
| − | <li>Redid E.Coli Cadmium Tolerance Test in M9 media
| + | <td>13</td> |
| − | <li>Learned to do cell count for AMB-1
| + | <td>14</td> |
| − | <li>Attempted to make AMB-1 chemically competent
| + | <td>15</td> |
| − | | + | <td>16</td> |
| − | <li>Human Practices
| + | <td>17</td> |
| − | <li>Presented to high school students at Penn M&T program
| + | </tr> |
| − | <li>Planned a synthetic biology preceptorial
| + | <tr> |
| − | <li>Contacted Schuylkill Action Network
| + | <td>18</td> |
| − | </ol>
| + | <td>19</td> |
| − | <h3 style= "text-align: center;">Week 10</h3>
| + | <td>20</td> |
| − | <ol> | + | <td>21</td> |
| − | <li>Completed AMB-1 growth curve under different media
| + | <td class="appoint">22</td> |
| − | <li>Determined cell count and OD600 conversion formola for AMB-1 experimentally
| + | <td class="appoint">23</td> |
| − | <li>Completed E.Coli Cadmium Tolerance Test in LB media
| + | <td>24</td> |
| − | | + | </tr> |
| − | <li>Human Practices
| + | <tr> |
| − | <li> Volunteered at SEA Science Carnival
| + | <td>25</td> |
| − | </ol>
| + | <td>26</td> |
| − | <h3 style= "text-align: center;">Week 11</h3>
| + | <td>27</td> |
| − | <ol> | + | <td>28</td> |
| − | <li>Addressed EMSGM growth problem with pH
| + | <td>29</td> |
| − | <li>Make new recovery media with adjusted pH for optimal AMB-1 growth
| + | <td>30</td> |
| − | <li>Chemical Transformation with different recovery broths
| + | <td>31</td> |
| − | <li>Attempted PCR assembly with synthesized parts
| + | </tr> |
| − | </ol> | + | </table> |
| − | <h3 style= "text-align: center;">Week 12</h3> | + | </div> |
| − | <ol> | + | |
| − | <li>Used anaerobic chambers to grow plates
| + | |
| − | <li>Troubleshooted lack of magnetism with new iron maleate solution
| + | |
| − | <li>Troubleshooted AMB-1 electroporation experiment with plating at every step
| + | |
| − | <li>Began cloning successfolly assembled PCRed constructs into PYMB essentials
| + | |
| − | </ol> | + | |
| − | <h3 style= "text-align: center;">Week 13</h3> | + | |
| − | <ol>
| + | |
| − | <li>Finished cloning flurescent protein with smtA and mCherry into PYMB essentials
| + | |
| − | <li>Transform AMB-1 with this construct to test for successfol transformation with fluorescence test
| + | |
| − | </ol> | + | |
| − | <h3 style= "text-align: center;">Week 14</h3>
| + | |
| − | <ol>
| + | |
| − | <li>Make new E-MSGM media
| + | |
| − | <li>Re-designed construct
| + | |
| − | </ol> | + | |
| − | <h3 style= "text-align: center;">Week 15</h3> | + | |
| − | <ol> | + | |
| − | <li>Troubleshooted cloning E. coli construct for cadmium tolerance
| + | |
| − | <li>Sequence verified finished constructs on PYMB
| + | |
| − | </ol>
| + | |
| − | <h3 style= "text-align: center;">Week 16</h3>
| + | |
| − | <ol> | + | |
| − | <li>Built the prototype of a portable spectrophotometer for cell recovery experiment
| + | |
| − | <li>Designed primers for Gibson Assembly of final construct
| + | |
| − | | + | |
| − | <li>Human Practices
| + | |
| − | <li>Presented to Penn BE 100 lecture to intriduce synthetic biology to freshman bioengineers.
| + | |
| − | </ol>
| + | |
| − | <h3 style= "text-align: center;">Week 17</h3>
| + | |
| − | <ol> | + | |
| − | <li>Tested AMB-1 aerobic colture and anaerobic colture with magnetometer
| + | |
| − | <li>Troubleshooted and retried cloning E. coli construct
| + | |
| − | <li>PCRed up parts we received for construction of shuttle vector
| + | |
| − | </ol>
| + | |
| − | <h3 style= "text-align: center;">Week 18</h3>
| + | |
| − | <ol> | + | |
| − | <li>Tested AMB-1 aerobic colture and anaerobic colture with magnetometer
| + | |
| − | <li>Finished PCRing parts we received from IDT
| + | |
| − | </ol>
| + | |
| − | <h3 style= "text-align: center;">Week 19</h3>
| + | |
| − | <ol> | + | |
| − | <li> Measured the magnetic strength indicator T2 for various concentration of AMB-1 colture and attempted to develop a trendline
| + | |
| − | <li> Counted folly grown sample of aerobic AMB-1 and made a trendline converting OD to cell concentration
| + | |
| − | <li>Cloned all biobricks into PSB1C3 backbone
| + | |
| − | </ol> | + | |
| − | <h3 style= "text-align: center;">Week 20</h3> | + | |
| − | <ol> | + | |
| − | <li>compiled all data and graphics for wiki
| + | |
| − | | + | |
| − | | + | |
| − | </ol><div id="textbox">
| + | |
| − | | + | |
| − | <table border="1">
| + | |
| − | | + | |
| − | <tr>
| + | |
| − | <th colspan="7">December 2013</th>
| + | |
| − | </tr> | + | |
| − | | + | |
| − | <tr>
| + | |
| − | <th>Su</th>
| + | |
| − | <th>Mo</th>
| + | |
| − | <th>Tu</th>
| + | |
| − | <th>We</th>
| + | |
| − | <th>Th</th>
| + | |
| − | <th>Fr</th>
| + | |
| − | <th>Sa</th>
| + | |
| − | </tr>
| + | |
| − | | + | |
| − | <tr> | + | |
| − | <td>1</td> | + | |
| − | <td>2</td> | + | |
| − | <td>3</td> | + | |
| − | <td>4</td> | + | |
| − | <td>5</td> | + | |
| − | <td>6</td> | + | |
| − | <td>7</td> | + | |
| − | </tr> | + | |
| − | | + | |
| − | <tr> | + | |
| − | <td>8</td> | + | |
| − | <td>9</td> | + | |
| − | <td>10</td> | + | |
| − | <td>11</td> | + | |
| − | <td>12</td> | + | |
| − | <td>13</td> | + | |
| − | <td>14</td> | + | |
| − | </tr> | + | |
| − | | + | |
| − | <tr> | + | |
| − | <td>15</td> | + | |
| − | <td>16</td> | + | |
| − | <td>17</td> | + | |
| − | <td>18</td> | + | |
| − | <td>19</td> | + | |
| − | <td>20</td> | + | |
| − | <td>21</td> | + | |
| − | </tr> | + | |
| − | | + | |
| − | <tr> | + | |
| − | <td>22</td>
| + | |
| − | <td>23</td>
| + | |
| − | <td>24</td>
| + | |
| − | <td>25</td> | + | |
| − | <td>26</td> | + | |
| − | <td>27</td> | + | |
| − | <td>28</td> | + | |
| − | </tr>
| + | |
| − | | + | |
| − | <tr>
| + | |
| − | <td>29</td> | + | |
| − | <td>30</td> | + | |
| − | <td>31</td> | + | |
| − | </tr> | + | |
| − | | + | |
| − | </table> | + | |
| | </body> | | </body> |
| | </html> | | </html> |
