Difference between revisions of "Team:UNC-Chapel Hill"
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− | <h3 style="color:#56A0D3;"> | + | <h3 style="color:#56A0D3;">Motivation and Example System</h3> |
<p>Through the development of a novel glucose sensing system in which glucose-responsive promoters drive the expression of three reporter chromoproteins, it will allow for the quantization of glucose concentration based on fluorescence emitted by the cell. With each chromoprotein linked to a different promoter with unique glucose sensitivities, each wavelength will correspond to a specific concentration of glucose. In conjunction with preexisting parts, we designed four novel glucose-sensitive promoters and tested their ability to drive expression of reporter chromoproteins at various concentrations. | <p>Through the development of a novel glucose sensing system in which glucose-responsive promoters drive the expression of three reporter chromoproteins, it will allow for the quantization of glucose concentration based on fluorescence emitted by the cell. With each chromoprotein linked to a different promoter with unique glucose sensitivities, each wavelength will correspond to a specific concentration of glucose. In conjunction with preexisting parts, we designed four novel glucose-sensitive promoters and tested their ability to drive expression of reporter chromoproteins at various concentrations. | ||
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− | <h3 style="color:#56A0D3;"> | + | <h3 style="color:#56A0D3;">Project Aim</h3> |
<p>In order to turn our three-color glucose sensor into a more viable solution, more characterization of the system will be necessary. This will allow for better identification of concentrations based on the fluorescence. While our project’s intended application was the detection of glucose concentrations, we believe its greatest utility is in its versatility towards other sensing systems. By changing out the promoters, it will allow for the cell to become sensitive to other chemicals and be used to calculate concentration. | <p>In order to turn our three-color glucose sensor into a more viable solution, more characterization of the system will be necessary. This will allow for better identification of concentrations based on the fluorescence. While our project’s intended application was the detection of glucose concentrations, we believe its greatest utility is in its versatility towards other sensing systems. By changing out the promoters, it will allow for the cell to become sensitive to other chemicals and be used to calculate concentration. | ||
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Revision as of 00:40, 19 September 2015
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We will list all of our important sponsors here! |