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Revision as of 17:00, 28 August 2015
Flight iGEM
The official example template of creating an iGEM page with Fligh iGEM.
pNZ8148
乳酸杆菌表达载体
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.
pBBR-1-MCS-5
Mini-prep
Today I have condulted a mini-prep.However,the result is not satisfying at all,for the concentration of the plasmid are all no more than 100ng/μl.