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| − | <font size="6" face="Arimo">Method of Measurement</font>
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| − | <hr width="100%" >
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| − | <ul>
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| − | <font size="4" face="Arimo">
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| − | We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to <i>E. coli</i> K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were transferred to 96-well plate by 100mL. We read the absorbance.
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| − | <br>
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| − | Setting of plate reader is following:<br></font>
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| − | <img src="https://static.igem.org/mediawiki/2015/2/20/Set_safari_gify.png" border="0" width="395" height="220">
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| − | <br><br><br><br>
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| − | </ul>
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| − | <font size="6" face="Arimo">Result and discussion</font>
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| − | <hr width="100%" ><br>
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| − | <ul>
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| − | <li> <font size="5" face="Arimo"> Definition<br></font> </li>
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| − | <font size="4" face="Arimo">
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| − | We expressed absorbance as relative value for positive control. <br>This expression is following:<br></font>
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| − | <img src="https://static.igem.org/mediawiki/2015/8/85/Siki-gifu.png" border="0" width="211" height="57"><br>
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| − | Sample : Absorbance in sample<br>
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| − | NCav : Average of absorbance in negative control<br>
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| − | PCav : Average of absorbance in positive control<br>
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| − | <br><br><br>
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| − | <li> <font size="5" face="Arimo"> Value<br></font> </li>
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| − | <font size="4" face="Arimo">
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| − | Relative absorbance values for positive control are shown below. <br>
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| − | <img src="https://static.igem.org/mediawiki/2015/e/e4/GURAHU-gifu.png" border="0" width="572" height="364"> <br><br>
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| − |
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| − | </ul>
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Revision as of 07:09, 29 August 2015
