Difference between revisions of "Team:Pitt/Notebook"

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<hr>
 
<hr>
 +
<i>Note: all experiments were done with all members present, unless otherwise noted</i>
 +
<h4> June 15, 2015</h4>
 +
<table class="notebook">
 +
  <tr class="head">
 +
    <th>Protocol</th>
 +
    <th>Notes/results/purpose</th>
 +
  </tr>
 +
 
 +
  <tr> 
 +
    <td>Transformation of plasmids from Pardee paper</td>
 +
    <td>Into DH5a, all transformations successful</td>
 +
  </tr>
  
−
<h4> May 19, 2015: First day in lab </h4>
+
  <tr>  
−
<h5> Transformation of cells with various plasmids </h5>
+
    <td>Grow O/N of DH5a</td>
 +
    <td>Competent cell prep (Konstantin Borisov)</td>
 +
  </tr>
 +
</table>
 +
<hr>
 +
<br/>
 +
<h4> June 16, 2015</h4>
 
<table class="notebook">
 
<table class="notebook">
 
   <tr class="head">
 
   <tr class="head">
−
     <td>Protocol</td>
+
     <th>Protocol</th>
−
     <td>Notes/results</td>
+
     <th>Notes/results/purpose</th>
 
   </tr>
 
   </tr>
 
    
 
    
 
   <tr>   
 
   <tr>   
−
     <td>Show up in lab</td>
+
     <td>O/N of transformations</td>
−
     <td>Some people had trouble</td>
+
     <td>Miniprep</td>
 
   </tr>
 
   </tr>
  
 
   <tr>   
 
   <tr>   
−
     <td>Get dressed for lab</td>
+
     <td>DH5a competent cell prep</td>
−
     <td>Some people <i>still</i> had trouble</td>
+
     <td>25 tubes of 200ul each, enough to last all summer! (Konstantin Borisov, help from Tatyana Yatsenko)</td>
 +
  </tr>
 +
</table>
 +
<hr>
 +
<br/>
 +
<h4> June 17, 2015</h4>
 +
<table class="notebook">
 +
  <tr class="head">
 +
    <th>Protocol</th>
 +
    <th>Notes/results/purpose</th>
 +
  </tr>
 +
 
 +
  <tr> 
 +
    <td>Transformation of plasmids from iGEM registry</td>
 +
    <td>Into new DH5a, all transformations successful</td>
 +
  </tr>
 +
</table>
 +
<hr>
 +
<br/>
 +
<h4> June 18, 2015</h4>
 +
<table class="notebook">
 +
  <tr class="head">
 +
    <th>Protocol</th>
 +
    <th>Notes/results/purpose</th>
 +
  </tr>
 +
 
 +
  <tr> 
 +
    <td>Test PT7 lysate with Pardee paper plasmids</td>
 +
    <td>No convenient method of visualization, so we started looking for a plate reader</td>
 +
  </tr>
 +
</table>
 +
<hr>
 +
<br/>
 +
<h4> June 22, 2015</h4>
 +
<table class="notebook">
 +
  <tr class="head">
 +
    <th>Protocol</th>
 +
    <th>Notes/results/purpose</th>
 +
  </tr>
 +
 
 +
  <tr> 
 +
    <td>PCR pSB1C3 with backbone fwd and rev primers</td>
 +
    <td>For Gibson assembly, learning process</td>
 
   </tr>
 
   </tr>
  
 
   <tr>   
 
   <tr>   
−
     <td>Start the thing</td>
+
     <td>Gel of PCR products</td>
−
     <td></td>
+
     <td>Visualization and purification of pSB1C3 backbone</td>
 
   </tr>
 
   </tr>
 +
<tr><td>Gel purification</td><td>Obtained enough backbone for the rest of the summer!</td></tr>
 
</table>
 
</table>
 
<hr>
 
<hr>
−
<br>
+
<br/>
−
<br>
+
<h4> June 23, 2015</h4>
−
<br>
+
<table class="notebook">
−
 
+
  <tr class="head">
−
<p> Document the dates you worked on your project.</p>
+
    <th>Protocol</th>
−
 
+
    <th>Notes/results/purpose</th>
−
<h5>What should this page have?</h5>
+
  </tr>
−
<ul>
+
 
−
<li>Chronological notes of what your team is doing.</li>
+
  <tr>  
−
<li> Brief descriptions of daily important events.</li>
+
    <td>Gibson assembly of protease parts from IDT, transformation into DH5a</td>
−
<li>Pictures of your progress. </li>
+
    <td>All transformations multiple colonies</td>
−
<li>Mention who participated in what task.</li>
+
  </tr>
−
</ul>
+
  
 +
  <tr> 
 +
    <td>Transformation of CMU ERT7 into DH5a</td>
 +
    <td>Success, faint yellow from YFP seen under blue light</td>
 +
  </tr>
 +
</table>
 +
<hr>
 +
<br/>
 +
<h4> June 24, 2015</h4>
 +
<table class="notebook">
 +
  <tr class="head">
 +
    <th>Protocol</th>
 +
    <th>Notes/results/purpose</th>
 +
  </tr>
 +
 
 +
  <tr> 
 +
    <td>Miniprep and diagnostic gel of Gibson assembly</td>
 +
    <td>All 3 had appropriate bands</td>
 +
  </tr>
  
−
<h4>Inspiration</h4>
+
  <tr>  
−
<p>You can see what others teams have done to organize their notes:</p>
+
    <td>Crude lysate</td>
−
 
+
    <td>Original S30 extract protocol, much too dilute due to incorrect weighing</td>
−
<ul>
+
  </tr>
−
<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
+
</table>
−
<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
+
<hr>
−
<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
+
<br/>
−
<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
+
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Revision as of 23:22, 18 September 2015

Notebook

Note: all experiments were done with all members present, unless otherwise noted

June 15, 2015

Protocol Notes/results/purpose
Transformation of plasmids from Pardee paper Into DH5a, all transformations successful
Grow O/N of DH5a Competent cell prep (Konstantin Borisov)

June 16, 2015

Protocol Notes/results/purpose
O/N of transformations Miniprep
DH5a competent cell prep 25 tubes of 200ul each, enough to last all summer! (Konstantin Borisov, help from Tatyana Yatsenko)

June 17, 2015

Protocol Notes/results/purpose
Transformation of plasmids from iGEM registry Into new DH5a, all transformations successful

June 18, 2015

Protocol Notes/results/purpose
Test PT7 lysate with Pardee paper plasmids No convenient method of visualization, so we started looking for a plate reader

June 22, 2015

Protocol Notes/results/purpose
PCR pSB1C3 with backbone fwd and rev primers For Gibson assembly, learning process
Gel of PCR products Visualization and purification of pSB1C3 backbone
Gel purificationObtained enough backbone for the rest of the summer!

June 23, 2015

Protocol Notes/results/purpose
Gibson assembly of protease parts from IDT, transformation into DH5a All transformations multiple colonies
Transformation of CMU ERT7 into DH5a Success, faint yellow from YFP seen under blue light

June 24, 2015

Protocol Notes/results/purpose
Miniprep and diagnostic gel of Gibson assembly All 3 had appropriate bands
Crude lysate Original S30 extract protocol, much too dilute due to incorrect weighing