Difference between revisions of "Team:Toulouse/Experiments"

Revision as of 10:11, 1 September 2015

iGEM Toulouse 2015

YETM 500mL	TFB1 200mL	TFB2 200mL
2.5g Yeast Extract 10g Tryptone 5g MgSO4.7H2O Adjust pH to 7.5 with KOH For Plates: add 7.5g of Agar	0.59g KOAc 2.42g RbCl 0.29g CaCl2.2H2O 1.98g MnCl2.4H2O Adjust to pH 5.8 with 0.2 M acetic acid Add dH2O to 200mL Filter sterilize Store refrigerated at 4°C	0.42g MOPS 2.21g CaCl2.2H20 0.24g RbCl 30g Glycerol Adjust to pH 6.5 with KOH Add dH2O to 200mL Filter sterilize Store refrigerated at 4°C

References

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