Difference between revisions of "Team:Gifu/project/"
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<p> One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated complementary sequences around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved.</p> | <p> One was an efficiency of the circularization. The efficiency was lower in our previous study. This was why the splicing was hard to happen because two sequences to act as ribozyme for splicing were far each other. So we incorporated complementary sequences around the ribozyme regions. We thought that the treatment brought two regions close and the efficiency of the circularization improved.</p> | ||
<p> The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated linker sequences into circular mRNA to synthesize the functional long-chain protein.</p> | <p> The other was to synthesize useful proteins. In our previous study, synthesized long-chain proteins lose their function because the folding of the proteins was broken. So we incorporated linker sequences into circular mRNA to synthesize the functional long-chain protein.</p> | ||
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+ | </font> | ||
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+ | <a name="ASS" style="text-decoration:none;"></a> <br> | ||
+ | <hr width="100%" > | ||
+ | <h4> <font size="5" face="Century"> About Self-Splicing </font> </h4> | ||
+ | <font size="3"> | ||
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+ | <p> The group I intron in td gene of T4 phage has self-splicing mechanism. The self-splicing is a mechanism that circularizes the intron and connects exons. This is catalyzed by several base sequences of the ends of the introns as a ribozyme. We permuted exons and introns with the mechanism and attempted an exon circularization. So we constructed mRNA circularization devices in last year.</p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
</font> | </font> | ||
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<li><a href="#descri" style="text-decoration:none;" >DESCRIPTION </a></li> | <li><a href="#descri" style="text-decoration:none;" >DESCRIPTION </a></li> | ||
<li><a href="#abst"style="text-decoration:none;" >ABSTRACT </a></li> | <li><a href="#abst"style="text-decoration:none;" >ABSTRACT </a></li> | ||
− | <li><a href="# | + | <li><a href="#ASS"style="text-decoration:none;" >About Self-Splicing </a></li> |
<li><a href="#T&M"style="text-decoration:none;" >Theory and Methods </a></li> | <li><a href="#T&M"style="text-decoration:none;" >Theory and Methods </a></li> | ||
<li><a href="#experiments"style="text-decoration:none;" >Experiments </a></li> | <li><a href="#experiments"style="text-decoration:none;" >Experiments </a></li> |
Revision as of 02:08, 2 September 2015