Difference between revisions of "Team:Aalto-Helsinki/Experiments"
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p> | <p>Describe the experiments, research and protocols you used in your iGEM project.</p> | ||
| − | < | + | <h4>Preparations</h4> |
<ul> | <ul> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2015/0/05/Aalto-Helsinki_chem_competent_cells.pdf">Chemically Competent Cells</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/0/05/Aalto-Helsinki_chem_competent_cells.pdf">Chemically Competent Cells</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/9/9a/Aalto-Helsinki_chloramphenicol_plates.pdf">Chloramphenicol plates</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/9/9a/Aalto-Helsinki_chloramphenicol_plates.pdf">Chloramphenicol plates</a></p></li> |
| − | <li><a href"https://static.igem.org/mediawiki/2015/8/88/Aalto-Helsinki_cmc_iptg_plate.pdf">CMC Plates with IPTG</a></li> | + | <li><p><a href"https://static.igem.org/mediawiki/2015/8/88/Aalto-Helsinki_cmc_iptg_plate.pdf">CMC Plates with IPTG</a></p></li> |
| − | <li>CMC Plates with Congo Red</li> | + | <li><p>CMC Plates with Congo Red</p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/b/b5/Aalto-Helsinki_colony_pcr.pdf">Colony PCR</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b5/Aalto-Helsinki_colony_pcr.pdf">Colony PCR</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/8/83/Aalto-Helsinki_glycerol_stock.pdf">Glycerol Stock of Bacteria</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/8/83/Aalto-Helsinki_glycerol_stock.pdf">Glycerol Stock of Bacteria</a></p></li> |
| − | <li><a href="https://2015.igem.org/File:Aalto-Helsinki_kapa_pcr.pdf">PCR with KAPA</a></li> | + | <li><p><a href="https://2015.igem.org/File:Aalto-Helsinki_kapa_pcr.pdf">PCR with KAPA</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/a/a4/Aalto-Helsinki_q5_pcr.pdf">PCR with Q5</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/a/a4/Aalto-Helsinki_q5_pcr.pdf">PCR with Q5</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_sds-page_sample.pdf">SDS-PAGE</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_sds-page_sample.pdf">SDS-PAGE</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/b/b7/Aalto-Helsinki_transformation.pdf">Transformation</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b7/Aalto-Helsinki_transformation.pdf">Transformation</a></p></li> |
| − | <li>Tetracyclin plates</li> | + | <li><p>Tetracyclin plates</p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/e/ed/Aalto-Helsinki_terrific_broth.pdf">Terrific Broth Media</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/e/ed/Aalto-Helsinki_terrific_broth.pdf">Terrific Broth Media</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/0/0a/Aalto-Helsinki_topo_cloning.pdf">TOPO Cloning</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/0/0a/Aalto-Helsinki_topo_cloning.pdf">TOPO Cloning</a></p></li> |
</ul> | </ul> | ||
| − | < | + | <h4>Assembly</h4> |
<ul> | <ul> | ||
| − | <li>ELIC</li> | + | <li><p>ELIC</p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/4/42/Aalto-Helsinki_ligation_protocol.pdf">Ligation</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/4/42/Aalto-Helsinki_ligation_protocol.pdf">Ligation</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/7/72/Aalto-Helsinki_gibson_assembly.pdf">NEBuilder</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/7/72/Aalto-Helsinki_gibson_assembly.pdf">NEBuilder</a></p></li> |
| − | <li>OE-PCR</li> | + | <li><p>OE-PCR</p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_restriction_digestion.pdf">Restriction</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/c/c0/Aalto-Helsinki_restriction_digestion.pdf">Restriction</a></p></li> |
</ul> | </ul> | ||
| − | < | + | <h4>Measurements</h4> |
<ul> | <ul> | ||
| − | <li><a href="https://static.igem.org/mediawiki/2015/b/b0/Aalto-Helsinki_blue_chromoprotein.pdf">Blue Chromoprotein</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/b/b0/Aalto-Helsinki_blue_chromoprotein.pdf">Blue Chromoprotein</a></p></li> |
| − | <li><a href="https://static.igem.org/mediawiki/2015/4/4f/Aalto-Helsinki_cultivation_for_GC.pdf">Vial-Scale Propane Gas Chomatography</a></li> | + | <li><p><a href="https://static.igem.org/mediawiki/2015/4/4f/Aalto-Helsinki_cultivation_for_GC.pdf">Vial-Scale Propane Gas Chomatography</a></p></li> |
</ul> | </ul> | ||
| − | < | + | <!--<h3>Inspiration</h3> |
<ul> | <ul> | ||
<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li> | <li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li> | ||
<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li> | <li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li> | ||
<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li> | <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li> | ||
| − | </ul> | + | </ul>--> |
Revision as of 07:44, 4 September 2015
