Difference between revisions of "Team:HKUST-Rice/Potassium Sensor Modelling"
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<h1>Model's Assumption</h1> | <h1>Model's Assumption</h1> | ||
| − | <p></p> | + | <p>The effect of the endogenous Kdp system of E.coli was neglected.<br><br> |
| + | In our engineered E.coli, it contains the inserted plasmid of PKdpF plus green fluorescence protein generator (BBa_E0240) in pSB3K3 backbone as well as the endogenous Kdp operon, and both operons have the same promoter, PKdpF. As a matter of fact, titration by the endogenous kdp operon of the transcription inducer, phosphorylated KdpE which binds to PKdpF was expected initially. And the titration of phosphorylated KdpE is anticipated to lower the expression of GFP. However, when the DNA copy number of both endogenous and the inserted operons were determined, it was found that the number of endogenous kdp operon is 10 times smaller than that of the inserted one: | ||
| + | </p> | ||
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Revision as of 05:04, 3 September 2015
Modelling
Model's Assumption
The effect of the endogenous Kdp system of E.coli was neglected.
In our engineered E.coli, it contains the inserted plasmid of PKdpF plus green fluorescence protein generator (BBa_E0240) in pSB3K3 backbone as well as the endogenous Kdp operon, and both operons have the same promoter, PKdpF. As a matter of fact, titration by the endogenous kdp operon of the transcription inducer, phosphorylated KdpE which binds to PKdpF was expected initially. And the titration of phosphorylated KdpE is anticipated to lower the expression of GFP. However, when the DNA copy number of both endogenous and the inserted operons were determined, it was found that the number of endogenous kdp operon is 10 times smaller than that of the inserted one:
