Difference between revisions of "Team:OUC-China"
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+ | <h2>Platform for Electromagnetic Control</h2> | ||
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− | <h2 class="text- | + | <h2 class="text-uppercase"> |
− | <p | + | how does it <span>work ?</span> |
− | + | </h2> | |
− | + | <p> | |
+ | This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal. | ||
</p> | </p> | ||
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− | <div class="col- | + | <div class="row work-content"> |
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+ | <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive"> | ||
+ | <h3 class="text-uppercase text-center">step 1</h3> | ||
+ | <p>Our Magthermocoli contains 2 core components: <B>magnetic receiver & thermosensitive regulator.</B></p> | ||
+ | </div> | ||
+ | <div class="col-md-4"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive"> | ||
+ | <h3 class="text-uppercase text-center">step 2</h3> | ||
+ | <p> | ||
+ | Induced by electromagnetic field,magnetic receiver would be heated, thus raise the ambient temperature. | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="col-md-4"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/6/6d/OUC-China_home_receiver_regulator3.png" alt="" class="img-responsive"> | ||
+ | <h3 class="text-uppercase text-center">step 3</h3> | ||
+ | <p> | ||
+ | In response to the change of temperature, thermosensitive regulator would initiate GFP (or RFP) expression. | ||
+ | </p> | ||
+ | </div> | ||
</div> | </div> | ||
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− | <div class=" | + | <div class="container-fluid index-details1 index-details"> |
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− | <div class="col- | + | <h3 class="text-center index-headline">Magnetic <span>Receiver</span></h3> |
− | < | + | <div class="row"> |
+ | <div class="col-md-6"> | ||
+ | <div class="caption"> | ||
+ | <p> | ||
+ | For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in organisms, which could synthesizeferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core would be heated, raising the ambient temperature. | ||
+ | </p> | ||
+ | <p> | ||
+ | In this session, we explored measurement techniques for the valuation of magnetism、biomineralization and Protein concentration per cell. | ||
+ | </p> | ||
+ | <p><a href="#" class="btn btn-primary" role="button">More details</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/d/d3/OUC-China-home_frritin.png" alt="" class="img-responsive"> | ||
+ | </div> | ||
</div> | </div> | ||
− | + | </div> | |
− | + | </div> | |
− | + | <div class="container-fluid index-details2 index-details"> | |
− | + | <div class="container"> | |
− | RNA thermometer is a structured RNA which could expose RBS only | + | <h3 class="text-center index-headline index-headline2">Thermosensitive <span>Regulator</span></h3> |
− | + | <p class="text-center">For thermosensitive regulator,we chose <B>RNA thermometer</B> and designed a <B>thermosensitive T7 RNA polymerase.</B> </p> | |
− | + | <div class="row"> | |
− | + | <div class="col-md-6"> | |
+ | <img src="https://static.igem.org/mediawiki/2015/c/cf/OUC-China-home_RNA.png" alt="" class="img-responsive"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <div class="caption"> | ||
+ | <p> | ||
+ | <B>RNA thermometer</B> is a structured RNA which could expose RBS only at appropriate temperature. In this session, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress. | ||
+ | </p> | ||
+ | <p><a href="#" class="btn btn-primary" role="button">More details</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <div class="caption"> | ||
+ | <p> | ||
+ | <B>Thermosensitive T7 RNA polymerase</B> is a normal T7 RNA polymerase at whose selected locus was inserted by a temperature-sensitive intein, which could self-splice at specific temperature. | ||
+ | </p> | ||
+ | <p><a href="#" class="btn btn-primary" role="button">More details</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/9/9e/OUC-China-home_T7.png" alt="" class="img-responsive"> | ||
+ | </div> | ||
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− | + | Follow us on Faceboo@ <a href="#">iGEM OUC</a> | |
− | </ | + | <br>mail to: <a href="mailto:#">oucigem@163.com</a> |
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Revision as of 15:49, 15 September 2015
<!DOCTYPE html>
how does it work ?
This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
![](https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png)
step 1
Our Magthermocoli contains 2 core components: magnetic receiver & thermosensitive regulator.
![](https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png)
step 2
Induced by electromagnetic field,magnetic receiver would be heated, thus raise the ambient temperature.
![](https://static.igem.org/mediawiki/2015/6/6d/OUC-China_home_receiver_regulator3.png)
step 3
In response to the change of temperature, thermosensitive regulator would initiate GFP (or RFP) expression.
Magnetic Receiver
For magnetic receiver,we chose Ferritin, the iron-storage protein in organisms, which could synthesizeferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core would be heated, raising the ambient temperature.
In this session, we explored measurement techniques for the valuation of magnetism、biomineralization and Protein concentration per cell.
![](https://static.igem.org/mediawiki/2015/d/d3/OUC-China-home_frritin.png)
Thermosensitive Regulator
For thermosensitive regulator,we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.
![](https://static.igem.org/mediawiki/2015/c/cf/OUC-China-home_RNA.png)
RNA thermometer is a structured RNA which could expose RBS only at appropriate temperature. In this session, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
Thermosensitive T7 RNA polymerase is a normal T7 RNA polymerase at whose selected locus was inserted by a temperature-sensitive intein, which could self-splice at specific temperature.
![](https://static.igem.org/mediawiki/2015/9/9e/OUC-China-home_T7.png)