Difference between revisions of "Team:York/Experiments"
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<h2>Experiments</h2> | <h2>Experiments</h2> | ||
<p>The following is a weekly description of the experiments carried out each week. To see the protocols click <a href="https://2015.igem.org/Team:York/Protocols">here</a></p> | <p>The following is a weekly description of the experiments carried out each week. To see the protocols click <a href="https://2015.igem.org/Team:York/Protocols">here</a></p> | ||
| + | |||
| + | <h3>Dry Lab Work Period</h3> | ||
| + | <ul> | ||
| + | <li>28/01: Brief introductory meeting about the iGEM competition | ||
| + | <li> 17/03: The official iGEM team to represent the University of York was formed! | ||
| + | <li>05/05: Article published in Nouse (University newspaper) about the 2015 iGEM team. | ||
| + | <li>03/06: We were invited to Science and Technology Alumni Network discussion event (outreach) | ||
| + | <li>04/06: Article published on the University's central news about this years iGEM team. | ||
| + | <li>12/06: A meeting was held with Yorkshire Water to discuss water remediation processes in the Yorkshire region | ||
| + | <li>13/06: We were invited to a University of York Biology Alumni event | ||
| + | <li>15/06: | ||
| + | <ol> | ||
| + | <li>Some of us went to a round-table discussion about the impact of Santander at York as some of the team members won the International Connections Award by Santander.</li> | ||
| + | <li>Article published on Mind the Horizon about University of York iGEM</li></ol></li> | ||
| + | |||
| + | </ul> | ||
| + | |||
<h3>Week 1 - June 23-27 </h3> | <h3>Week 1 - June 23-27 </h3> | ||
<ul> | <ul> | ||
| − | + | ||
| − | + | ||
| − | + | ||
</ul> | </ul> | ||
<h3>Week 2 - June 29- July 3 </h3> | <h3>Week 2 - June 29- July 3 </h3> | ||
<ul> | <ul> | ||
| − | <li> | + | <li>Competent cells made</li> |
| + | <li>Plates were streaked with two isolates of each deletion wanted from the Keio collection [PPX, PPK, PstA, PstB, PstC, PstS and PhoE] These have been collected and put in the incubating (37°C) room.</li> | ||
| + | <li>Competent cells were tested with iGEM transformation efficiency kit</li> | ||
</ul> | </ul> | ||
<h3>Week 3 - July 6-10 </h3> | <h3>Week 3 - July 6-10 </h3> | ||
<ul> | <ul> | ||
| + | <li>More agar plates made, next set of competent cell procedure started</li> | ||
| + | <li>YUfund page done and submitted</li> | ||
</ul> | </ul> | ||
<h3>Week 4 - July 13-17 </h3> | <h3>Week 4 - July 13-17 </h3> | ||
<ul> | <ul> | ||
| + | <li>Vistited Badger Hill Primary School and held a bacteria workshop for the students</li> | ||
| + | <li>first day of demonstrations of cell transformations and aseptic technique to sixth formers</li> | ||
| + | <li>second day of demonstrations to sixth formers:analysing transformations and building bioreactors</li> | ||
| + | <Li>analysing bioreactors with sixth formers</li> | ||
| + | <li>first attempt at phosphate assay</li> | ||
</ul> | </ul> | ||
<h3>Week 5 - July 20-24 </h3> | <h3>Week 5 - July 20-24 </h3> | ||
<ul> | <ul> | ||
| + | <li>Phosphate assay was started for KEIO collection</li> | ||
| + | <li>Colony PCR on SmPstSCAB, EcPstSCAB and EcPhoE</li> | ||
| + | <li>Next set of competent cells made </li> | ||
| + | <li>Visit to AgeUK LunchClub to explain about the GM</li> | ||
</ul> | </ul> | ||
<h3>Week 6 - July 27-31 </h3> | <h3>Week 6 - July 27-31 </h3> | ||
<ul> | <ul> | ||
| + | <li>First day of outreaching event to Saint Helen's CHurch, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM </li> | ||
| + | <li>Second (and the last day) of outreaching event to Saint Helen's Church, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM </li> | ||
| + | <li>After several repeats the phosphate assay team managed to get significant data</li> | ||
</ul> | </ul> | ||
<h3>Week 7 - August 3-7 </h3> | <h3>Week 7 - August 3-7 </h3> | ||
<ul> | <ul> | ||
| + | <li>Plasmid (pBC 29 Keasling collection )loss experiment initiated</li> | ||
</ul> | </ul> | ||
Revision as of 09:48, 8 September 2015
Experiments
The following is a weekly description of the experiments carried out each week. To see the protocols click here
Dry Lab Work Period
- 28/01: Brief introductory meeting about the iGEM competition
- 17/03: The official iGEM team to represent the University of York was formed!
- 05/05: Article published in Nouse (University newspaper) about the 2015 iGEM team.
- 03/06: We were invited to Science and Technology Alumni Network discussion event (outreach)
- 04/06: Article published on the University's central news about this years iGEM team.
- 12/06: A meeting was held with Yorkshire Water to discuss water remediation processes in the Yorkshire region
- 13/06: We were invited to a University of York Biology Alumni event
- 15/06:
- Some of us went to a round-table discussion about the impact of Santander at York as some of the team members won the International Connections Award by Santander.
- Article published on Mind the Horizon about University of York iGEM
Week 1 - June 23-27
Week 2 - June 29- July 3
- Competent cells made
- Plates were streaked with two isolates of each deletion wanted from the Keio collection [PPX, PPK, PstA, PstB, PstC, PstS and PhoE] These have been collected and put in the incubating (37°C) room.
- Competent cells were tested with iGEM transformation efficiency kit
Week 3 - July 6-10
- More agar plates made, next set of competent cell procedure started
- YUfund page done and submitted
Week 4 - July 13-17
- Vistited Badger Hill Primary School and held a bacteria workshop for the students
- first day of demonstrations of cell transformations and aseptic technique to sixth formers
- second day of demonstrations to sixth formers:analysing transformations and building bioreactors
- analysing bioreactors with sixth formers
- first attempt at phosphate assay
Week 5 - July 20-24
- Phosphate assay was started for KEIO collection
- Colony PCR on SmPstSCAB, EcPstSCAB and EcPhoE
- Next set of competent cells made
- Visit to AgeUK LunchClub to explain about the GM
Week 6 - July 27-31
- First day of outreaching event to Saint Helen's CHurch, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM
- Second (and the last day) of outreaching event to Saint Helen's Church, York, to carry out strawberry extraction practical and briefly explain about the steps involved for GM
- After several repeats the phosphate assay team managed to get significant data
Week 7 - August 3-7
- Plasmid (pBC 29 Keasling collection )loss experiment initiated