Difference between revisions of "Team:Amoy/Project/Results"
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<p id="title_p">RESULTS</p> | <p id="title_p">RESULTS</p> | ||
| − | <h1 class="main_h1"> | + | <h1 class="main_h1">Ⅱ. Enzyme and protein assays</h1> |
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| + | <h2 class="main_h2">1. IPTG Gradient Induction</h2> | ||
<p class="main_p"></br>In order to make a better expression of the protein in the cell, we decided to make a IPTG gradient induction for our gene circuits after the reference. Here ares our rusults.</p> | <p class="main_p"></br>In order to make a better expression of the protein in the cell, we decided to make a IPTG gradient induction for our gene circuits after the reference. Here ares our rusults.</p> | ||
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<h1 class="col-md-12" style="border-bottom: 1px dashed #aaaaaa; margin-bottom: 40px;"></h1> | <h1 class="col-md-12" style="border-bottom: 1px dashed #aaaaaa; margin-bottom: 40px;"></h1> | ||
| − | <p class="main_p"></br>From the enzyme activity data, we obtained the most suitable IPTG concentration of different circuits, as follows.</br></br> | + | <p class="main_p"></br>From the enzyme activity data, we obtained the most suitable IPTG concentration of different circuits, as follows.</br></br></p> |
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| + | <h2 class="main_h2">2. HPLC</h2> | ||
| − | When we get the most appropriate concentration, we start with High Performance Liquid Chromatography </p> | + | <p class="main_p">When we get the most appropriate concentration, we start with High Performance Liquid Chromatography </p> |
<img class="main_img" src="https://static.igem.org/mediawiki/2015/8/8b/Amoy-Project_Result_figure2.14.png" style="width: 50%;" /> | <img class="main_img" src="https://static.igem.org/mediawiki/2015/8/8b/Amoy-Project_Result_figure2.14.png" style="width: 50%;" /> | ||
Revision as of 09:58, 9 September 2015
RESULTS
Ⅱ. Enzyme and protein assays
1. IPTG Gradient Induction
In order to make a better expression of the protein in the cell, we decided to make a IPTG gradient induction for our gene circuits after the reference. Here ares our rusults.
Figure 2.1 SDS-PAGE of purified LeuDH (34+L) from DE3
Figure 2.2 Different concentrations of IPTG on the Enzyme activity of 34+L
Figure 2.3 SDS-PAGE of purified FDH(34+F) from DE3
Figure 2.4 Different concentrations of IPTG on the Enzyme activity of 34+F
Figure 2.5 SDS-PAGE of purified LeuDH (30+L)from DE3
Figure 2.6 Different concentrations of IPTG on the Enzyme activity of 30+L
Figure 2.7 SDS-PAGE of purified LeuDH(30+L) and FDH(34+F) from DE3
Figure 2.8 Different concentrations of IPTG on the Enzyme activity of 30+L+34+
Figure 2.9 SDS-PAGE of purified LeuDH (34+L)and FDH (34+L)from DE3
Figure 2.10 Different concentrations of IPTG on the Enzyme activity of 34+L+34+F
Figure 2.11 SDS-PAGE of purified LeuDH(32+L) and FDH(34+F) from DE3
Figure 2.12 Different concentrations of IPTG on the Enzyme activity of 32+L+34+F
From the enzyme activity data, we obtained the most suitable IPTG concentration of different circuits, as follows.
2. HPLC
When we get the most appropriate concentration, we start with High Performance Liquid Chromatography
CONTACT US
Email: igemxmu@gmail.com
Website: 2015.igem.org/Team:Amoy
Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005
